Team:Lyon-INSA-ENS/Realisation/Collection

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(Difference between revisions)

Revision as of 15:09, 14 September 2011

Plasmids collection
Collection reference	Description	Resistance(s)
pIG1	phaCAB	-
pIG2	rcn-curli cloned in pUC57 plasmid	Amp
pIG3	Pfort (18A: BBa_J23119)	Amp
pIG4	part Bba_K342000 = Pcurli	Cm
pIG5	enhanced yellow fluorescent protein derived from A. victoria GFP (24E: BBa_E0030)	Amp+K
pIG6	pUC18	Amp
pIG7	pUC18	Amp
pIG8	Weak RBS (5L: Bba_J61101)	Amp
pIG9	GFP with LVA tag (2L: BBa_K145015)	Amp
pIG10	RBS-YFP (22M: BBa_I15017)	Amp+K
pIG11	OmpR234 (BBa_K342003)	Cm
pIG13	RBS-GFP-LVA (BBa_K206009)	Cm
pIG14	Strong RBS (2M: BBa_B0034)	Amp
pIG15	NiCoT	Amp
pIG16	18A-OmpR234 (pIG3+pIG11)	Amp
pIG17	Strong RBS-YFP (pIG14+pIG10) (2M24E)	Amp
pIG18	RBSfort-GFP-Tet (pIG14+pIG9) (2M2L)	T
pIG19	RBSfort-GFP-Kan (pIG14+pIG9) (2M2L)	K
pIG20	Prcn-csg extracted in pUC57 ()	Amp
pIG21	Backbone A = pSB1A3	Amp
pIG22	Backbone K = pSB1K3	K
pIG23	Backbone T = pSB1T3	T
pIG24	Backbone C = pSB1C3	Cm
pIG25	Synthetic part cloned in pSB1C3	Cm
pIG26	part Prcn (= rcnA promoter)	Cm
pIG27	part Prcn (= rcnA promoter)	Cm
pIG28	part Pcurli-RBS-GFP(LVA) (pIG4+pIG18)	Cm
pIG29	part CsgBA	Amp
pIG30	pIG25	-
pIG31	pKK-OmpR234 (p56 collection biofilm)	Amp
pIG32	pPROBE OT'-Prcn-GFP (p157 collection biofilm)	Spc
pIG33	PrcnA-OmpR234	Cm
pIG34	PrcnA-OmpR234	K
pIG35	= pIG25	Cm

Strains collection
Collection reference	Description	Phenotype
S5		Amp resistant
S6	NM522/pIG26
S7	MC4100
S8	MG1655
S9	NM522/pIG27
S10	NM522/pIG26
S11	NM522
S12	DH5α
S13	NMYO
S14	NM522 + pTNiCo
S15	MG1655 malT::Tn10 ompR234
S16	MG1655 malT::Tn10 ompR234/ p127 (fusion PcsgBA-GFP-LVA on pPROBE)
S17	F' proA+B+ lacIq Δ(lacZ)M15/ Δ(lac-proAB) glnV thi-1 Δ(hsdS-mcrB)5/ pIG25 = synthetic part rcn-curli, cloned in the backbone pBS1C3
S18	F' proA+B+ lacIq Δ(lacZ)M15/ Δ(lac-proAB) glnV thi-1 Δ(hsdS-mcrB)5/ pIG2
S19	F' proA+B+ lacIq Δ(lacZ)M15/ Δ(lac-proAB) glnV thi-1 Δ(hsdS-mcrB)5/ pIG16
S21	F' proA+B+ lacIq Δ(lacZ)M15/ Δ(lac-proAB) glnV thi-1 Δ(hsdS-mcrB)5/ pUC18
S22	F' proA+B+ lacIq Δ(lacZ)M15/ Δ(lac-proAB) glnV thi-1 Δ(hsdS-mcrB)5/ pIG33
S23	Strain derivated form MG1655 expressing constitutively GFP with pIG2 plasmid
S24	Strain derivated form MG1655 expressing constitutively GFP with pIG6 plasmid
S25	F- [araD139]B/r Δ(argF-lac)169* &lambda- e14- flhD5301 Δ(fruK-yeiR)725 (fruA25)‡ relA1 rpsL150(strR) rbsR22 Δ(fimB-fimE)632(::IS1) deoC1/pIG6
S26	F- [araD139]B/r Δ(argF-lac)169* &lambda- e14- flhD5301 Δ(fruK-yeiR)725 (fruA25)‡ relA1 rpsL150(strR) rbsR22 Δ(fimB-fimE)632(::IS1) deoC1/pIG2
S27
S28
S29

@@ Line 172: / Line 172: @@
 </tr>
 <tr>
-<td>S5</td> <td>NM522/pGem-T with rcnR inserted</td> <td> Amp resistant </td>
+<td>S5</td> <td></td> <td> Amp resistant </td>
 </tr>
 <tr>
@@ Line 214: / Line 214: @@
 </tr>
 <tr>
-<td>S19</td> <td></td> <td> </td>
+<td>S19</td> <td>F' proA+B+ lacIq Δ(lacZ)M15/ Δ(lac-proAB) glnV thi-1 Δ(hsdS-mcrB)5/ pIG16</td> <td> </td>
 </tr>
-<tr>
-<td>S20</td> <td></td> <td> </td>
-</tr>
 <tr>
-<td>S21</td> <td></td> <td> </td>
+<td>S21</td> <td>F' proA+B+ lacIq Δ(lacZ)M15/ Δ(lac-proAB) glnV thi-1 Δ(hsdS-mcrB)5/ pUC18</td> <td> </td>
 </tr>
 <tr>
-<td>S22</td> <td></td> <td> </td>
+<td>S22</td> <td>F' proA+B+ lacIq Δ(lacZ)M15/ Δ(lac-proAB) glnV thi-1 Δ(hsdS-mcrB)5/ pIG33</td> <td> </td>
 </tr>
 <tr>
-<td>S23</td> <td></td> <td> </td>
+<td>S23</td> <td>Strain derivated form MG1655 expressing constitutively GFP with pIG2 plasmid</td> <td> </td>
 </tr>
 <tr>
-<td>S24</td> <td></td> <td> </td>
+<td>S24</td> <td>Strain derivated form MG1655 expressing constitutively GFP with pIG6 plasmid</td> <td> </td>
 </tr>
 <tr>
-<td>S25</td> <td></td> <td> </td>
+<td>S25</td> <td>F- [araD139]B/r Δ(argF-lac)169* &lambda- e14- flhD5301 Δ(fruK-yeiR)725 (fruA25)‡ relA1 rpsL150(strR) rbsR22 Δ(fimB-fimE)632(::IS1) deoC1/pIG6</td> <td> </td>
 </tr>
 <tr>
-<td>S26</td> <td></td> <td> </td>
+<td>S26</td> <td>F- [araD139]B/r Δ(argF-lac)169* &lambda- e14- flhD5301 Δ(fruK-yeiR)725 (fruA25)‡ relA1 rpsL150(strR) rbsR22 Δ(fimB-fimE)632(::IS1) deoC1/pIG2</td> <td> </td>
 </tr>
 <tr>