Team:Lyon-INSA-ENS/Realisation/Collection

From 2011.igem.org

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Revision as of 15:00, 14 September 2011






Plasmids collection
Collection reference Description Resistance(s)
pIG1 phaCAB -
pIG2 rcn-curli cloned in pUC57 plasmid Amp
pIG3 Pfort (18A: BBa_J23119) Amp
pIG4 part Bba_K342000 = Pcurli Cm
pIG5 enhanced yellow fluorescent protein derived from A. victoria GFP (24E: BBa_E0030) Amp+K
pIG6 pUC18 Amp
pIG7 pUC18 Amp
pIG8 Weak RBS (5L: Bba_J61101) Amp
pIG9 GFP with LVA tag (2L: BBa_K145015) Amp
pIG10 RBS-YFP (22M: BBa_I15017) Amp+K
pIG11 OmpR234 (BBa_K342003) Cm
pIG13 RBS-GFP-LVA (BBa_K206009) Cm
pIG14 Strong RBS (2M: BBa_B0034) Amp
pIG15 NiCoT Amp
pIG16 18A-OmpR234 (pIG3+pIG11) Amp
pIG17 Strong RBS-YFP (pIG14+pIG10) (2M24E) Amp
pIG18 RBSfort-GFP-Tet (pIG14+pIG9) (2M2L) T
pIG19 RBSfort-GFP-Kan (pIG14+pIG9) (2M2L) K
pIG20 Prcn-csg extracted in pUC57 () Amp
pIG21 Backbone A = pSB1A3 Amp
pIG22 Backbone K = pSB1K3 K
pIG23 Backbone T = pSB1T3 T
pIG24 Backbone C = pSB1C3 Cm
pIG25 Synthetic part cloned in pSB1C3 Cm
pIG26 part Prcn (= rcnA promoter) Cm
pIG27 part Prcn (= rcnA promoter) Cm
pIG28 part Pcurli-RBS-GFP(LVA) (pIG4+pIG18) Cm
pIG29 part CsgBA Amp
pIG30 pIG25 -
pIG31 pKK-OmpR234 (p56 collection biofilm) Amp
pIG32 pPROBE OT'-Prcn-GFP (p157 collection biofilm) Spc
pIG33 PrcnA-OmpR234 Cm
pIG34 PrcnA-OmpR234 K
pIG35 = pIG25 Cm




Strains collection
Collection reference Description Phenotype
S5 NM522/pGem-T with rcnR inserted Amp resistant
S6 NM522/pIG26
S7 MC4100
S8 MG1655
S9 NM522/pIG27
S10 NM522/pIG26
S11 NM522
S12 DH5α
S13 NMYO
S14 NM522 + pTNiCo
S15 MG1655 malT::Tn10 ompR234
S16
S17
S18
S19
S20
S21
S22
S23
S24
S25
S26
S27
S28
S29





ENS assystem Biomérieux INSA INSA