Team:Lyon-INSA-ENS/Realisation/Collection

From 2011.igem.org

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<td>S5</td> <td>NM522/pGem-T with rcnR inserted</td> <td> Amp resistant </td>
<td>S5</td> <td>NM522/pGem-T with rcnR inserted</td> <td> Amp resistant </td>
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<td>S6</td> <td></td> <td> </td>
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<td>S7</td> <td></td> <td> </td>
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<td>S8</td> <td></td> <td> </td>
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<td>S9</td> <td></td> <td> </td>
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<td>S10</td> <td></td> <td> </td>
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<td>S11</td> <td></td> <td> </td>
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<td>S12</td> <td></td> <td> </td>
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<td>S13</td> <td></td> <td> </td>
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<td>S14</td> <td></td> <td> </td>
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<td>S15</td> <td></td> <td> </td>
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<td>S16</td> <td></td> <td> </td>
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<td>S17</td> <td></td> <td> </td>
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<td>S18</td> <td></td> <td> </td>
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<td>S19</td> <td></td> <td> </td>
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<td>S20</td> <td></td> <td> </td>
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<td>S21</td> <td></td> <td> </td>
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<td>S22</td> <td></td> <td> </td>
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<td>S23</td> <td></td> <td> </td>
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<td>S24</td> <td></td> <td> </td>
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<td>S25</td> <td></td> <td> </td>
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<td>S26</td> <td></td> <td> </td>
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<td>S27</td> <td></td> <td> </td>
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<td>S28</td> <td></td> <td> </td>
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<td>S29</td> <td></td> <td> </td>
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Revision as of 14:39, 14 September 2011






Plasmids collection
Collection reference Description Resistance(s)
pIG1 phaCAB -
pIG2 rcn-curli cloned in pUC57 plasmid Amp
pIG3 Pfort (18A: BBa_J23119) Amp
pIG4 part Bba_K342000 = Pcurli Cm
pIG5 enhanced yellow fluorescent protein derived from A. victoria GFP (24E: BBa_E0030) Amp+K
pIG6 pUC18 Amp
pIG7 pUC18 Amp
pIG8 Weak RBS (5L: Bba_J61101) Amp
pIG9 GFP with LVA tag (2L: BBa_K145015) Amp
pIG10 RBS-YFP (22M: BBa_I15017) Amp+K
pIG11 OmpR234 (BBa_K342003) Cm
pIG13 RBS-GFP-LVA (BBa_K206009) Cm
pIG14 Strong RBS (2M: BBa_B0034) Amp
pIG15 NiCoT Amp
pIG16 18A-OmpR234 (pIG3+pIG11) Amp
pIG17 Strong RBS-YFP (pIG14+pIG10) (2M24E) Amp
pIG18 RBSfort-GFP-Tet (pIG14+pIG9) (2M2L) T
pIG19 RBSfort-GFP-Kan (pIG14+pIG9) (2M2L) K
pIG20 Prcn-csg extracted in pUC57 () Amp
pIG21 Backbone A = pSB1A3 Amp
pIG22 Backbone K = pSB1K3 K
pIG23 Backbone T = pSB1T3 T
pIG24 Backbone C = pSB1C3 Cm
pIG25 Synthetic part cloned in pSB1C3 Cm
pIG26 part Prcn (= rcnA promoter) Cm
pIG27 part Prcn (= rcnA promoter) Cm
pIG28 part Pcurli-RBS-GFP(LVA) (pIG4+pIG18) Cm
pIG29 part CsgBA Amp
pIG30 pIG25 -
pIG31 pKK-OmpR234 (p56 collection biofilm) Amp
pIG32 pPROBE OT'-Prcn-GFP (p157 collection biofilm) Spc
pIG33 PrcnA-OmpR234 Cm
pIG34 PrcnA-OmpR234 K
pIG35 = pIG25 Cm




Strains collection
Collection reference Description Phenotype
S5 NM522/pGem-T with rcnR inserted Amp resistant
S6
S7
S8
S9
S10
S11
S12
S13
S14
S15
S16
S17
S18
S19
S20
S21
S22
S23
S24
S25
S26
S27
S28
S29





ENS assystem Biomérieux INSA INSA