Team:Lyon-INSA-ENS/Realisation/Collection

From 2011.igem.org

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<td>pIG1</td> <td>phaCAB</td> <td> </td>
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<td>pIG1</td> <td>phaCAB</td> <td> - </td>
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<td>pIG2</td> <td>rcn-curli cloned in pUC57 plasmid</td> <td> </td>
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<td>pIG2</td> <td>rcn-curli cloned in pUC57 plasmid</td> <td> A </td>
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<td>pIG3</td> <td>Pfort (18A: BBa_J23119)</td> <td> </td>
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<td>pIG3</td> <td>Pfort (18A: BBa_J23119)</td> <td> A </td>
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<td>pIG4</td> <td>part Bba_K342000 = Pcurli</td> <td> </td>
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<td>pIG4</td> <td>part Bba_K342000 = Pcurli</td> <td> C </td>
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<td>pIG5</td> <td>enhanced yellow fluorescent protein derived from A. victoria GFP (24E: BBa_E0030)</td> <td> </td>
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<td>pIG5</td> <td>enhanced yellow fluorescent protein derived from A. victoria GFP (24E: BBa_E0030)</td> <td> AK </td>
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<td>pIG6</td> <td>pUC18</td> <td> </td>
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<td>pIG6</td> <td>pUC18</td> <td> A </td>
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<td>pIG7</td> <td>pUC18</td> <td> </td>
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<td>pIG7</td> <td>pUC18</td> <td> A </td>
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<td>pIG8</td> <td>RBS faible (5L: Bba_J61101) </td> <td> </td>
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<td>pIG8</td> <td>RBS faible (5L: Bba_J61101) </td> <td> A </td>
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<td>pIG9</td> <td>GFP with LVA tag (2L: BBa_K145015)</td> <td> </td>
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<td>pIG9</td> <td>GFP with LVA tag (2L: BBa_K145015)</td> <td> A </td>
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<td>pIG10</td> <td>RBS-YFP (22M: BBa_I15017) </td> <td> </td>
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<td>pIG10</td> <td>RBS-YFP (22M: BBa_I15017) </td> <td> AK </td>
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<td>pIG11</td> <td>OmpR234 (BBa_K342003)</td> <td> </td>
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<td>pIG11</td> <td>OmpR234 (BBa_K342003)</td> <td> C </td>
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<td>pIG12</td> <td>GFP ?</td> <td> </td>
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<td>pIG12</td> <td>GFP ?</td> <td> - </td>
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<td>pIG13</td> <td>RBS-GFP-LVA (BBa_K206009)</td> <td> </td>
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<td>pIG13</td> <td>RBS-GFP-LVA (BBa_K206009)</td> <td> C </td>
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<td>pIG14</td> <td>Strong RBS (2M: BBa_B0034)</td> <td> </td>
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<td>pIG14</td> <td>Strong RBS (2M: BBa_B0034)</td> <td> A </td>
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<td>pIG15</td> <td>NiCoT</td> <td> </td>
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<td>pIG15</td> <td>NiCoT</td> <td> A </td>
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<td>pIG16</td> <td>18A-OmpR234 (pIG3+pIG11)</td> <td> </td>
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<td>pIG16</td> <td>18A-OmpR234 (pIG3+pIG11)</td> <td> A </td>
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<td>pIG17</td> <td>Strong RBS-YFP (pIG14+pIG10) (2M24E)</td> <td> </td>
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<td>pIG17</td> <td>Strong RBS-YFP (pIG14+pIG10) (2M24E)</td> <td> A </td>
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<td>pIG18</td> <td>RBSfort-GFP-Tet (pIG14+pIG9) (2M2L)</td> <td> </td>
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<td>pIG18</td> <td>RBSfort-GFP-Tet (pIG14+pIG9) (2M2L)</td> <td> T </td>
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<td>pIG19</td> <td>RBSfort-GFP-Kan (pIG14+pIG9) (2M2L)</td> <td> </td>
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<td>pIG19</td> <td>RBSfort-GFP-Kan (pIG14+pIG9) (2M2L)</td> <td> K </td>
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<td>pIG20</td> <td>Prcn-csg extrait dans pUC57 (à confirmer, différence avec la pIG2?)</td> <td> </td>
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<td>pIG20</td> <td>Prcn-csg extrait dans pUC57 (à confirmer, différence avec la pIG2?)</td> <td> A </td>
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<td>pIG21</td> <td>Backbone A = pSB1A3</td> <td> </td>
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<td>pIG21</td> <td>Backbone A = pSB1A3</td> <td> A </td>
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<td>pIG22</td> <td>Backbone K = pSB1K3</td> <td> </td>
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<td>pIG22</td> <td>Backbone K = pSB1K3</td> <td> K </td>
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<td>pIG23</td> <td>Backbone T = pSB1T3</td> <td> </td>
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<td>pIG23</td> <td>Backbone T = pSB1T3</td> <td> T </td>
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<td>pIG24</td> <td>Backbone C = pSB1C3</td> <td> </td>
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<td>pIG24</td> <td>Backbone C = pSB1C3</td> <td> C </td>
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<td>pIG25</td> <td>part de synthèse clonée dans pSB1C3</td> <td> </td>
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<td>pIG25</td> <td>part de synthèse clonée dans pSB1C3</td> <td> C </td>
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<td>pIG26</td> <td>part Prcn (= promoter rcnA) </td> <td> </td>
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<td>pIG26</td> <td>part Prcn (= promoter rcnA) </td> <td> C </td>
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<td>pIG27</td> <td>part Prcn (= promoter rcnA)</td> <td> </td>
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<td>pIG27</td> <td>part Prcn (= promoter rcnA)</td> <td> C </td>
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<td>pIG28</td> <td>part Pcurli-RBS-GFP(LVA) (pIG4+pIG18)</td> <td> </td>
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<td>pIG28</td> <td>part Pcurli-RBS-GFP(LVA) (pIG4+pIG18)</td> <td> C </td>
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<td>pIG29</td> <td>part CsgBA</td> <td> </td>
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<td>pIG29</td> <td>part CsgBA</td> <td> A </td>
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<td>pIG30</td> <td>pIG25</td> <td> </td>
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<td>pIG30</td> <td>pIG25</td> <td> - </td>
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<td>pIG31</td> <td>pKK-OmpR234 (p56 collection biofilm)</td> <td> </td>
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<td>pIG31</td> <td>pKK-OmpR234 (p56 collection biofilm)</td> <td> A </td>
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<td>pIG32</td> <td>pPROBE OT'-Prcn-GFP (p157 collection biofilm)</td> <td> </td>
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<td>pIG32</td> <td>pPROBE OT'-Prcn-GFP (p157 collection biofilm)</td> <td> Spc </td>
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<td>pIG33</td> <td>PrcnA-OmpR234</td> <td> </td>
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<td>pIG33</td> <td>PrcnA-OmpR234</td> <td> Cm </td>
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<td>pIG34</td> <td>PrcnA-OmpR234</td> <td> </td>
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<td>pIG34</td> <td>PrcnA-OmpR234</td> <td> K </td>
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<td>pIG35</td> <td>= pIG25</td> <td> </td>
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<td>pIG35</td> <td>= pIG25</td> <td> Cm </td>
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Revision as of 13:43, 14 September 2011






Collection reference Description Resistance(s)
pIG1 phaCAB -
pIG2 rcn-curli cloned in pUC57 plasmid A
pIG3 Pfort (18A: BBa_J23119) A
pIG4 part Bba_K342000 = Pcurli C
pIG5 enhanced yellow fluorescent protein derived from A. victoria GFP (24E: BBa_E0030) AK
pIG6 pUC18 A
pIG7 pUC18 A
pIG8 RBS faible (5L: Bba_J61101) A
pIG9 GFP with LVA tag (2L: BBa_K145015) A
pIG10 RBS-YFP (22M: BBa_I15017) AK
pIG11 OmpR234 (BBa_K342003) C
pIG12 GFP ? -
pIG13 RBS-GFP-LVA (BBa_K206009) C
pIG14 Strong RBS (2M: BBa_B0034) A
pIG15 NiCoT A
pIG16 18A-OmpR234 (pIG3+pIG11) A
pIG17 Strong RBS-YFP (pIG14+pIG10) (2M24E) A
pIG18 RBSfort-GFP-Tet (pIG14+pIG9) (2M2L) T
pIG19 RBSfort-GFP-Kan (pIG14+pIG9) (2M2L) K
pIG20 Prcn-csg extrait dans pUC57 (à confirmer, différence avec la pIG2?) A
pIG21 Backbone A = pSB1A3 A
pIG22 Backbone K = pSB1K3 K
pIG23 Backbone T = pSB1T3 T
pIG24 Backbone C = pSB1C3 C
pIG25 part de synthèse clonée dans pSB1C3 C
pIG26 part Prcn (= promoter rcnA) C
pIG27 part Prcn (= promoter rcnA) C
pIG28 part Pcurli-RBS-GFP(LVA) (pIG4+pIG18) C
pIG29 part CsgBA A
pIG30 pIG25 -
pIG31 pKK-OmpR234 (p56 collection biofilm) A
pIG32 pPROBE OT'-Prcn-GFP (p157 collection biofilm) Spc
pIG33 PrcnA-OmpR234 Cm
pIG34 PrcnA-OmpR234 K
pIG35 = pIG25 Cm





ENS assystem Biomérieux INSA INSA