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Team:Lyon-INSA-ENS/Realisation/Collection
From 2011.igem.org
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| - | <td>pIG1</td> <td>phaCAB</td> <td> | + | <td>pIG1</td> <td>phaCAB</td> <td> - </td> |
</tr> | </tr> | ||
| - | <td>pIG2</td> <td>rcn-curli cloned in pUC57 plasmid</td> <td> | + | <td>pIG2</td> <td>rcn-curli cloned in pUC57 plasmid</td> <td> A </td> |
</tr> | </tr> | ||
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| - | <td>pIG3</td> <td>Pfort (18A: BBa_J23119)</td> <td> | + | <td>pIG3</td> <td>Pfort (18A: BBa_J23119)</td> <td> A </td> |
</tr> | </tr> | ||
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| - | <td>pIG4</td> <td>part Bba_K342000 = Pcurli</td> <td> | + | <td>pIG4</td> <td>part Bba_K342000 = Pcurli</td> <td> C </td> |
</tr> | </tr> | ||
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| - | <td>pIG5</td> <td>enhanced yellow fluorescent protein derived from A. victoria GFP (24E: BBa_E0030)</td> <td> | + | <td>pIG5</td> <td>enhanced yellow fluorescent protein derived from A. victoria GFP (24E: BBa_E0030)</td> <td> AK </td> |
</tr> | </tr> | ||
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| - | <td>pIG6</td> <td>pUC18</td> <td> | + | <td>pIG6</td> <td>pUC18</td> <td> A </td> |
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| - | <td>pIG7</td> <td>pUC18</td> <td> | + | <td>pIG7</td> <td>pUC18</td> <td> A </td> |
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| - | <td>pIG8</td> <td>RBS faible (5L: Bba_J61101) </td> <td> | + | <td>pIG8</td> <td>RBS faible (5L: Bba_J61101) </td> <td> A </td> |
</tr> | </tr> | ||
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| - | <td>pIG9</td> <td>GFP with LVA tag (2L: BBa_K145015)</td> <td> | + | <td>pIG9</td> <td>GFP with LVA tag (2L: BBa_K145015)</td> <td> A </td> |
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| - | <td>pIG10</td> <td>RBS-YFP (22M: BBa_I15017) </td> <td> | + | <td>pIG10</td> <td>RBS-YFP (22M: BBa_I15017) </td> <td> AK </td> |
</tr> | </tr> | ||
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| - | <td>pIG11</td> <td>OmpR234 (BBa_K342003)</td> <td> | + | <td>pIG11</td> <td>OmpR234 (BBa_K342003)</td> <td> C </td> |
</tr> | </tr> | ||
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| - | <td>pIG12</td> <td>GFP ?</td> <td> | + | <td>pIG12</td> <td>GFP ?</td> <td> - </td> |
</tr> | </tr> | ||
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| - | <td>pIG13</td> <td>RBS-GFP-LVA (BBa_K206009)</td> <td> | + | <td>pIG13</td> <td>RBS-GFP-LVA (BBa_K206009)</td> <td> C </td> |
</tr> | </tr> | ||
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| - | <td>pIG14</td> <td>Strong RBS (2M: BBa_B0034)</td> <td> | + | <td>pIG14</td> <td>Strong RBS (2M: BBa_B0034)</td> <td> A </td> |
</tr> | </tr> | ||
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| - | <td>pIG15</td> <td>NiCoT</td> <td> | + | <td>pIG15</td> <td>NiCoT</td> <td> A </td> |
</tr> | </tr> | ||
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| - | <td>pIG16</td> <td>18A-OmpR234 (pIG3+pIG11)</td> <td> | + | <td>pIG16</td> <td>18A-OmpR234 (pIG3+pIG11)</td> <td> A </td> |
</tr> | </tr> | ||
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| - | <td>pIG17</td> <td>Strong RBS-YFP (pIG14+pIG10) (2M24E)</td> <td> | + | <td>pIG17</td> <td>Strong RBS-YFP (pIG14+pIG10) (2M24E)</td> <td> A </td> |
</tr> | </tr> | ||
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| - | <td>pIG18</td> <td>RBSfort-GFP-Tet (pIG14+pIG9) (2M2L)</td> <td> | + | <td>pIG18</td> <td>RBSfort-GFP-Tet (pIG14+pIG9) (2M2L)</td> <td> T </td> |
</tr> | </tr> | ||
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| - | <td>pIG19</td> <td>RBSfort-GFP-Kan (pIG14+pIG9) (2M2L)</td> <td> | + | <td>pIG19</td> <td>RBSfort-GFP-Kan (pIG14+pIG9) (2M2L)</td> <td> K </td> |
</tr> | </tr> | ||
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| - | <td>pIG20</td> <td>Prcn-csg extrait dans pUC57 (à confirmer, différence avec la pIG2?)</td> <td> | + | <td>pIG20</td> <td>Prcn-csg extrait dans pUC57 (à confirmer, différence avec la pIG2?)</td> <td> A </td> |
</tr> | </tr> | ||
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| - | <td>pIG21</td> <td>Backbone A = pSB1A3</td> <td> | + | <td>pIG21</td> <td>Backbone A = pSB1A3</td> <td> A </td> |
</tr> | </tr> | ||
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| - | <td>pIG22</td> <td>Backbone K = pSB1K3</td> <td> | + | <td>pIG22</td> <td>Backbone K = pSB1K3</td> <td> K </td> |
</tr> | </tr> | ||
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| - | <td>pIG23</td> <td>Backbone T = pSB1T3</td> <td> | + | <td>pIG23</td> <td>Backbone T = pSB1T3</td> <td> T </td> |
</tr> | </tr> | ||
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| - | <td>pIG24</td> <td>Backbone C = pSB1C3</td> <td> | + | <td>pIG24</td> <td>Backbone C = pSB1C3</td> <td> C </td> |
</tr> | </tr> | ||
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| - | <td>pIG25</td> <td>part de synthèse clonée dans pSB1C3</td> <td> | + | <td>pIG25</td> <td>part de synthèse clonée dans pSB1C3</td> <td> C </td> |
</tr> | </tr> | ||
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| - | <td>pIG26</td> <td>part Prcn (= promoter rcnA) </td> <td> | + | <td>pIG26</td> <td>part Prcn (= promoter rcnA) </td> <td> C </td> |
</tr> | </tr> | ||
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| - | <td>pIG27</td> <td>part Prcn (= promoter rcnA)</td> <td> | + | <td>pIG27</td> <td>part Prcn (= promoter rcnA)</td> <td> C </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>pIG28</td> <td>part Pcurli-RBS-GFP(LVA) (pIG4+pIG18)</td> <td> | + | <td>pIG28</td> <td>part Pcurli-RBS-GFP(LVA) (pIG4+pIG18)</td> <td> C </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>pIG29</td> <td>part CsgBA</td> <td> | + | <td>pIG29</td> <td>part CsgBA</td> <td> A </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>pIG30</td> <td>pIG25</td> <td> | + | <td>pIG30</td> <td>pIG25</td> <td> - </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>pIG31</td> <td>pKK-OmpR234 (p56 collection biofilm)</td> <td> | + | <td>pIG31</td> <td>pKK-OmpR234 (p56 collection biofilm)</td> <td> A </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>pIG32</td> <td>pPROBE OT'-Prcn-GFP (p157 collection biofilm)</td> <td> | + | <td>pIG32</td> <td>pPROBE OT'-Prcn-GFP (p157 collection biofilm)</td> <td> Spc </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>pIG33</td> <td>PrcnA-OmpR234</td> <td> | + | <td>pIG33</td> <td>PrcnA-OmpR234</td> <td> Cm </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>pIG34</td> <td>PrcnA-OmpR234</td> <td> | + | <td>pIG34</td> <td>PrcnA-OmpR234</td> <td> K </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>pIG35</td> <td>= pIG25</td> <td> | + | <td>pIG35</td> <td>= pIG25</td> <td> Cm </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
Revision as of 13:43, 14 September 2011
| Collection reference | Description | Resistance(s) |
|---|---|---|
| pIG1 | phaCAB | - | pIG2 | rcn-curli cloned in pUC57 plasmid | A |
| pIG3 | Pfort (18A: BBa_J23119) | A |
| pIG4 | part Bba_K342000 = Pcurli | C |
| pIG5 | enhanced yellow fluorescent protein derived from A. victoria GFP (24E: BBa_E0030) | AK |
| pIG6 | pUC18 | A |
| pIG7 | pUC18 | A |
| pIG8 | RBS faible (5L: Bba_J61101) | A |
| pIG9 | GFP with LVA tag (2L: BBa_K145015) | A |
| pIG10 | RBS-YFP (22M: BBa_I15017) | AK |
| pIG11 | OmpR234 (BBa_K342003) | C |
| pIG12 | GFP ? | - |
| pIG13 | RBS-GFP-LVA (BBa_K206009) | C |
| pIG14 | Strong RBS (2M: BBa_B0034) | A |
| pIG15 | NiCoT | A |
| pIG16 | 18A-OmpR234 (pIG3+pIG11) | A |
| pIG17 | Strong RBS-YFP (pIG14+pIG10) (2M24E) | A |
| pIG18 | RBSfort-GFP-Tet (pIG14+pIG9) (2M2L) | T |
| pIG19 | RBSfort-GFP-Kan (pIG14+pIG9) (2M2L) | K |
| pIG20 | Prcn-csg extrait dans pUC57 (à confirmer, différence avec la pIG2?) | A |
| pIG21 | Backbone A = pSB1A3 | A |
| pIG22 | Backbone K = pSB1K3 | K |
| pIG23 | Backbone T = pSB1T3 | T |
| pIG24 | Backbone C = pSB1C3 | C |
| pIG25 | part de synthèse clonée dans pSB1C3 | C |
| pIG26 | part Prcn (= promoter rcnA) | C |
| pIG27 | part Prcn (= promoter rcnA) | C |
| pIG28 | part Pcurli-RBS-GFP(LVA) (pIG4+pIG18) | C |
| pIG29 | part CsgBA | A |
| pIG30 | pIG25 | - |
| pIG31 | pKK-OmpR234 (p56 collection biofilm) | A |
| pIG32 | pPROBE OT'-Prcn-GFP (p157 collection biofilm) | Spc |
| pIG33 | PrcnA-OmpR234 | Cm |
| pIG34 | PrcnA-OmpR234 | K |
| pIG35 | = pIG25 | Cm |
