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Team:Washington/Protocols/Colony
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Revision as of 22:44, 13 September 2011
Colony PCR with Green tag
Master mix(7ul):
1ul 10uM forword primer
1ul 10uM reverse Primer
5ul 2x Green tag
Cell water(3ul): Pick one colony from the plate and mix with 10ul of ddH2O to make 10ul of cell water
Reaction = Master mix(7ul) + Cell water(3ul) = 10ul per tube
Use program "Colony" & change the extention time (1min per kb)
