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Team:Washington/Protocols/Ligation
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Revision as of 21:53, 13 September 2011
General Ligation Protocol
7uL insert
1uL vector
1uL T4 ligase buffer
1uL T4 ligase
incubate at 37C no. **Note that NEB website recommends 16C - room temp for ligations, we do ours at RT and it works well. 1 hour.
