Team:UT-Tokyo/LabNote

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Revision as of 20:20, 13 September 2011

Parts List

Table 1. List of iGEM parts
Number Part Content Plasmid Length (bp)
#1 J23119 c.Promoter (Strong) pSB1A2 35
#2 J23118 c.Promoter (Medium) BBa_J61002 35
#3 B0032 RBS pSB1A2 13
#5 B0014 d.Ter pSB1AK3 95
#9 E0240 RBS-GFP-d.Ter pSB1A2 876
#10 E0040 GFP pSB1A2 681
#11 E1010 RFP pSB2K3 723
#14 I712019 fLuc pSB1AK8 1653
#17 J52008 rLuc pSB1AK3 936
#20 R0011 lacP pSB1A2 55
#21 C0012 LacI pSB1A2 1128
#22 I712074 pT7 pSB1AK8 46
#23 K145001 T7 RNA Pol. pSB1A2 2655
#24 J22106 recAp pSB1A2 192
#27 C0083 AspA pSB2K3 1518
#28 K112808 T4 phage lysis device (no promoter) pSB1A2 1785
#29 - CheZ pSB1AK3 728
#30 K117000 Lysis gene pSB1A2 144
#31 - LexA pSB1AK3 750
#33 - sulAp pSB1AK3 67
#34 - uvrAp pSB1AK3 96
#35 - recNp
#36 R0051 cI-repressed promoter pSB1A2 49
#37 C0051 cI repressor (LVA tagged) pSB1A2 \
#38 - RecA
#2-9 c.Promoter (M)-RBS-GFP-d.Ter pSB1A2 911
#20-9 lacP-RBS-GFP-d.Ter pSB1A2 931
#22-9 pT7-RBS-GFP-d.Ter pSB1AK8 922
#3-11 RBS-RFP pSB1A2 736
#3-11-5 RBS-RFP-d.Ter pSB1AK3 831
pSB1C3 831
#2-3-11-5 c.Promoter(M)-RBS-RFP-d.Ter pSB1A2 866
pSB1AK3 866
#24-3-11-5 recAp-RBS-RFP-d.Ter pSB1A2 1023
#33-3-11-5 sulAp-RBS-RFP-d.Ter pSB1AK3 898
#14-5 fLuc-d.Ter pSB1AK3 1748
#3-14-5 RBS-ｆLuc-d.Ter pSB1A2 1761
#2-3-14-5 c.Promoter (M)-RBS-fLuc-d.Ter pSB1A2 1796
#20-3-14-5 lacP-RBS-fLuc-d.Ter pSB1A2 1816
#34-3-14-5 uvrAp-RBS-fLuc-d.Ter pSB1AK3 1857
#36-3-14-5 cIp-RBS-fLuc-d.Ter pSB1A2 1810
#17-5 ｒLuc-d.Ter pSB1AK3 1031
#3-17-5 RBS-ｒLuc-d.Ter pSB1A2 1044
#2-3-17-5 c.Promoter (M)-RBS-ｒLuc-d.Ter pSB1A2 1079
#24-3-17-5 recAp-RBS-rLuc-d.Ter pSB1A2 1236
#34-3-17-5 uvrAp-RBS-rLuc-d.Ter pSB1AK3 1140
#20-3-17-5 lacP-RBS-ｒLuc-d.Ter pSB1A2 1099
#3-14-5-2-3-17-5 RBS-ｆLuc-d.Ter-c.Promoter (M)-RBS-ｒLuc-d.Ter pSB1A2 2840
#20-3-14-5-2-3-17-5 lacP-RBS-ｆLuc-d.Ter-c.Promoter (M)-RBS-ｒLuc-d.Ter pSB1A2 2895
#36-3-14-5-2-3-17-5 cIp-RBS-ｆLuc-d.Ter-c.Promoter (M)-RBS-ｒLuc-d.Ter pSB1A2 2889
#3-27 RBS-AspA pSB1A2 1531
#3-27-5 RBS-AspA-d.Ter pSB1AK3 1626
#20-3-27-5 lacP-RBS-AspA-d.Ter pSB1A2 1681
#3-29 RBS-CheZ pSB1A2 741
#3-29-5 RBS-CheZ-d.Ter pSB1AK3 836
#20-3-29-5 lacP-RBS-CheZ-d.Ter pSB1A2 891
#36-3-29-5 cIp-RBS-CheZ-d.Ter ｐSB1A2 885
#37-5 cI-d.Ter pSB1AK3 #VALUE!
#3-37-5 RBS-cI-d.Ter pSB1A2 #VALUE!
#20-3-37-5 lacP-RBS-cI-d.Ter pSB1A2 #VALUE!
#20-28 lacP-〈T4 phage lysis device〉 pSB1A2 1840
#3-30 RBS-Lysis gene pSB1A2 157
#3-23 RBS-T7 RNA Pol. pSB1A2 2668
pSB1A2 Plasmid backbone pSB1A2 2079
pSB1AK3 Plasmid backbone pSB1AK3 3189
pSB1A3 Linearized plasmid backbone pSB1A3 2155
pSB1K3 Linearized plasmid backbone pSB1K3 2204
pSB1C3 Linearized plasmid backbone pSB1C3 2070
pSB1C3 J04450 lacP-RBS-RFP-d.Ter pSB1C3 1069


Lab Diary

For convenience sake, each part (gene, promotors, etc) are represented by consecutive numbers (e.g. #20 for lac promotor). By pointing the mouse on the part number in the labnote, you can find out the details of the part.


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