Team:Osaka/week7

From 2011.igem.org

(Difference between revisions)
(→September 12(Mon))
Line 19: Line 19:
==September 13(Tue)==
==September 13(Tue)==
-
 
+
# Miniprep of yesterday's culture
 +
# Restriction digests of minipreppped parts
 +
# Gel electrophoresis of digests
 +
# Ligation
 +
#* 107(A):12(upstream)+103(downstream)+1-1G(vector)
 +
#* 108(A):12(upstream)+104(downstream)+1-1G(vector)
 +
# Transformation of ligation products
==September 14(Wed)==
==September 14(Wed)==

Revision as of 09:43, 13 September 2011


Contents

September 11(Sun)

  1. Gel extraction of digests
  2. Miniprep of yesterday's culture
  3. Restriction digests of minipreppped parts
  4. Gel electrophoresis of digests
  5. Ligation
    • 103(C):PprM+1-3A(vector)
    • 104(C):RecA+1-3A(vector)
    • 105(A):12(upstream)+101(downstream)+1-1G(vector)
    • 106(A):12(upstream)+102(downstream)+1-1G(vector)
  6. Transformation of ligation products


September 12(Mon)

  1. To liquid culture:103,104,105,106

September 13(Tue)

  1. Miniprep of yesterday's culture
  2. Restriction digests of minipreppped parts
  3. Gel electrophoresis of digests
  4. Ligation
    • 107(A):12(upstream)+103(downstream)+1-1G(vector)
    • 108(A):12(upstream)+104(downstream)+1-1G(vector)
  5. Transformation of ligation products

September 14(Wed)

September 15(Thu)

September 16(Fri)

September 17(Sat)

Back to Notebook

Retrieved from "http://2011.igem.org/Team:Osaka/week7"