"
Team:Paris Liliane Bettencourt/Notebook/2011/09/08/
From 2011.igem.org
(Difference between revisions)
(→Cyrille) |
(→Digestion and gel) |
||
| Line 16: | Line 16: | ||
Then the gel was runned and the bands cutted. | Then the gel was runned and the bands cutted. | ||
| - | [[File:CP0909_S24.jpg|thumb|S24 digested - S24a 1,2,3,4 - S24 b 1, 2,3, b - S24c 1,2,3,4]] | + | [[File:CP0909_S24.jpg|thumb|center|S24 digested - S24a 1,2,3,4 - S24 b 1, 2,3, b - S24c 1,2,3,4]] |
| - | [[File:CP9090_S24_cutted.jpg|thumb|S24 bands cutted]] | + | [[File:CP9090_S24_cutted.jpg|thumb|center|S24 bands cutted]] |
| - | [[File:CP0909_inserts.jpg|thumb|RFP Ter 1,2,3 - T7 amber ter 1,2,3 - KinA-ter 1,2,3]] | + | [[File:CP0909_inserts.jpg|thumb|center|RFP Ter 1,2,3 - T7 amber ter 1,2,3 - KinA-ter 1,2,3]] |
| - | [[File:CP0909_RFPTer.jpg|thumb|RFP ter cutted]] | + | [[File:CP0909_RFPTer.jpg|thumb|center|RFP ter cutted]] |
| - | [[File:CP0909_T7ter.jpg|thumb|T7 ter cutted]] | + | [[File:CP0909_T7ter.jpg|thumb|center|T7 ter cutted]] |
| - | [[File:CP0909_KinAter.jpg|thumb|KinA ter cutted]] | + | [[File:CP0909_KinAter.jpg|thumb|center|KinA ter cutted]] |
Then I proceed to a gel extraction of the bands. | Then I proceed to a gel extraction of the bands. | ||
Revision as of 09:51, 9 September 2011
Contents |
Cyrille
Digestion and gel
S24 is prepared to be cloned with
- RFP - TT
- T7 amber - TT
- ComS
- KinA-TT
3*4 500ng of S24 was digested and runned on the gel The insert was digested several time.
Then the gel was runned and the bands cutted.
Then I proceed to a gel extraction of the bands.
PCR colony of TetO/TetR
New attempt of PCR colony to find one good clone. Runned on a gel
Miniprep of YFP-TetR BB
Miniprep of the 3 last clones of YFP-TetR BB (that were not sequenced or red)
