"
Team:EPF-Lausanne/Todo
From 2011.igem.org
(Difference between revisions)
(→Wiki) |
(→Assembly) |
||
| Line 16: | Line 16: | ||
== Assembly == | == Assembly == | ||
| - | * Research plasmid backbones. Is there one that already contains Tet-repressed LacI or GFP? | + | * <s> Research plasmid backbones. Is there one that already contains Tet-repressed LacI or GFP? </s> |
* Design Gibson primers to assemble the three different plasmids. | * Design Gibson primers to assemble the three different plasmids. | ||
* Determine which sequence on LacI-plasmid and Lysis-plasmid should be used to create the "mega-plasmid". | * Determine which sequence on LacI-plasmid and Lysis-plasmid should be used to create the "mega-plasmid". | ||
Revision as of 15:05, 6 June 2011
Todo
Contents |
Microfluidic Chips
- Continue alignment training
Preparing the parts
- Investigate failure of lysis device sequencing.
Next steps:
-
Make competent cells -
Test competent cells~10^5CFU/µg not very good -
Transform in E. Coli. - Glycerol Stocks
-
Plasmid preps - Sequence the lysis cassette
Assembly
-
Research plasmid backbones. Is there one that already contains Tet-repressed LacI or GFP? - Design Gibson primers to assemble the three different plasmids.
- Determine which sequence on LacI-plasmid and Lysis-plasmid should be used to create the "mega-plasmid".
Wiki
For anybody:
- Start uploading protocols
- Write-up team presentation
Clean rooms
- Order lab notebook
- Order storage box
