Revision as of 13:38, 5 September 2011

Data

Description: The T7 promoter upstream of the RFP gene, set in the pSB3K1 backbone

Sequence:

Plasmid Map:

Saturation Curves:

Dose-Response Curves:

Plasmid backbone: pSB3K1 from ETHZ 2007 [http://partsregistry.org/wiki/index.php?title=Part:pSB3K1 "pSB3K1"] (taken from the delivery plate)
Pconst: J23116 from Berkeley 2006 [http://partsregistry.org/Part:BBa_J23116 "j23116"] (sequence copied into our primers)
RBS and spacer: (sequence copied into our primers)
TetR: "TetR sequence" (623 bp) The sequence lacks a stop codon, we added TAA with our primers

Sequenced data compared to the sequence of Pconst,RBS+spacer and TetR gene:"pSb3K1_TetR_seq" All these parts are correct.

Parts assembled

Plasmid backbone containing Pconst and TetR: see precedent section
Terminator: B0014 from the Registry [http://partsregistry.org/Part:BBa_B0014 "B0014"] (sequence copied into our primers)
Ptet: R0040 from Registry [http://partsregistry.org/Part:BBa_R0040 "R0040"] (sequence copied into our primers)
LacI: amplified from Repressilator plasmid "LacI sequence" The sequence lacks a stop codon, we added TAA with our primers.

Sequencing results:

@@ Line 11: / Line 11: @@
 * pSB3K1-T7-const-RFP
+''Description:'' The T7 promoter upstream of the RFP gene, set in the pSB3K1 backbone
+''Sequence:''
+''Plasmid Map:''
+''Saturation Curves:''
+''Dose-Response Curves:''
 * pSB3K1-T7-lac-RFP
 * pSB3K1-T7-lac2-RFP
 * pSB3K1-T7-const-Lysis
-* pSB3K1-T7-lac-Lysis
-* pSB3C5-T7-const-RFP
+* pSB3K1-T7-lac-Lysis
-* pSB3C5-T7-lac-RFP
-* pSB3C5-T7-lac2-RFP
-* pSB3C5-T7-lac-Lysis
-* pSB3C5-T7-lac2-Lysis
 == T7 Promoter Variants ==