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Team:EPF-Lausanne/Todo
From 2011.igem.org
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== Clean rooms == | == Clean rooms == | ||
Revision as of 20:32, 3 June 2011
Todo
Contents |
Microfluidic Chips
- Continue alignment training
Preparing the parts
- Investigate failure of lysis device sequencing.
Next steps:
-
Make competent cells -
Test competent cells~10^5CFU/µg not very good -
Transform in E. Coli. - Glycerol Stocks
-
Plasmid preps - Sequence the lysis cassette
Assembly
- Research plasmid backbones. Is there one that already contains Tet-repressed LacI or GFP?
- Design Gibson primers to assemble the three different plasmids.
- Determine which sequence on LacI-plasmid and Lysis-plasmid should be used to create the "mega-plasmid".
Wiki
For anybody:
- Start uploading protocols
- Write-up team presentation
Clean rooms
- Order lab notebook
- Order storage box
