Revision as of 13:22, 3 June 2011

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Next steps:

Research plasmid backbones. Is there one that already contains Tet-repressed LacI or GFP?
Design Gibson primers to assemble the three different plasmids.
Determine which sequence on LacI-plasmid and Lysis-plasmid should be used to create the "mega-plasmid".

For anybody:

For an HTML-literate person:

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 == Preparing the parts ==
-We need to sequence-verify the lysis brick and LacI.
+* Investigate failure of lysis device sequencing.
-'''Status''': Primers for sequencing the lysis brick have been ordered; reaction solutions for LacI sequencing have been prepared (ready to send out?).
 Next steps: