Team:EPF-Lausanne/Our Project/Data
From 2011.igem.org
(Difference between revisions)
(→pSB3K1 Pconst-TetR) |
|||
Line 34: | Line 34: | ||
Parts assembled: | Parts assembled: | ||
- | * backbone: pSB3K1 from ETHZ 2007 | + | * Plasmid backbone: pSB3K1 from ETHZ 2007 [http://partsregistry.org/wiki/index.php?title=Part:pSB3K1] (taken from the delivery plate) |
- | * Pconst: J23116 from Berkeley 2001 | + | * Pconst: J23116 from Berkeley 2001 [http://partsregistry.org/Part:BBa_J23116] (sequence copied into our primers) |
- | * RBS and spacer | + | * RBS and spacer: (sequence copied into our primers) |
+ | * TetR: from Alina? | ||
{{:Team:EPF-Lausanne/Templates/Footer}} | {{:Team:EPF-Lausanne/Templates/Footer}} |
Revision as of 15:59, 2 September 2011
Data
Contents |
TetR Mutants
- V36F
- P39K
- Y42F
- P39Q-Y42M
T7 Promoter Plasmids
- pSB3K1-T7-const-RFP
- pSB3K1-T7-lac-RFP
- pSB3K1-T7-lac2-RFP
- pSB3K1-T7-const-Lysis
- pSB3K1-T7-lac-Lysis
- pSB3C5-T7-const-RFP
- pSB3C5-T7-lac-RFP
- pSB3C5-T7-lac2-RFP
- pSB3C5-T7-lac-Lysis
- pSB3C5-T7-lac2-Lysis
T7 Promoter Variants
Reporter Plasmids
J61002 Ptet-RFP
TetR plasmids
pSB3K1 Pconst-TetR
Parts assembled:
- Plasmid backbone: pSB3K1 from ETHZ 2007 [http://partsregistry.org/wiki/index.php?title=Part:pSB3K1] (taken from the delivery plate)
- Pconst: J23116 from Berkeley 2001 [http://partsregistry.org/Part:BBa_J23116] (sequence copied into our primers)
- RBS and spacer: (sequence copied into our primers)
- TetR: from Alina?