Team:UQ-Australia/Safety

From 2011.igem.org

(Difference between revisions)
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<!-- ------------------------------------------------From 2011 Macquarie--------------------------------------------- -->
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{{:Team:UQ-Australia/Template:Header}}
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<body>
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<body style="background-color:#772288">
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</body>
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<head>
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<style type="text/css">
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body {background-color:#A11F37;}
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<!-- Wiki Hacks -->
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{
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overflow: -moz-scrollbars-vertical !important;
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resize: none;
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#siteSub {
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}
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height: 20px;
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border-right: none;
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}
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margin-top:-12px;
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margin-top:-12px;
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#menubar li a {
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color:#8C8984;
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        background-color:transparent;
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}
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#menubar ul {
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color:transparent;
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        background-color:transparent;
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}
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#menubar {
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background-color:transparent;
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font-size:85%;
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line-height:1em;
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position:absolute;
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top:10px;
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white-space:nowrap;
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width:50%;
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z-index:5;
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height:20px;
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overflow:hidden
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}
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.left-menu {
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left:0;
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padding-left: 0px;
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}
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.right-menu  {
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right:0;
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}
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.firstHeading { display:none; }
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}
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#menubar{
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color: #transparent;
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}
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#menubar.right-menu {
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margin-top:-12px;
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}
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.right-menu ul {
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border: none;
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}
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#menubar{
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top:15px;
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z-index:25;
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}
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#menubar {
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background-color:f5f6f7;
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font-size:85%;
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line-height:1em;
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position:absolute;
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top:10px;
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white-space:nowrap;
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width:50%;
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z-index:5;
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height:20px;
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overflow:hidden
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}
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img[src*="/wiki/skins/common/images/wiki.png"]{
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    width:0;
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    height:60px;
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    padding-right:975px;
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    background:#transparent;
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}
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ul#list-nav {
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list-style:none;
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margin:7px 75px 7px;
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padding:0;
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width:1000px
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}
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ul#list-nav li {
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display:inline
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}
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ul#list-nav li a {
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text-decoration:none;
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padding:5px 0;
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width:100px;
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background:#A11F37;
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color:#eee;
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float:left;
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text-align:center;
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border-left:1px solid #fff;
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}
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ul#list-nav li a:hover {
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background:#a2b3a1;
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color:#000
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}
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<!-- End of Wiki Hacks -->
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</body>
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</html>
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<!-- -------------------------------------------------From 2009 UQ--------------------------------------------------- -->
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__NOTOC__
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<html>
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<body style="background-color:#772288">
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<style>
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h1.firstHeading { display: none; }
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p {text-align: justify;}
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a:link { color: #603371; text-decoration: none}
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a:visited { color:#603371; text-decoration: none}
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a:hover { color:#f29400; text-decoration: none}
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a:active { color:#f29400; text-decoration: none}
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#bodyContent { padding: 10px auto; width: 910px; margin: auto; clear: none; }
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table#team_members { text-align: justify; border: 0; }
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table#team_members h2, table#team_members h3 { clear: both; }
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/*-----------------------------------------------------------------------------------------------*/
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div.MenuBar ul li ul.DropDownMenu {
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  display: none; /* Hides all drop-down menus. */
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}
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-
/*
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li:hover works in IE7 and FF2.
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a:hover works in IE6 and FF2.
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a:hover breaks li:hover in FF2.
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*/
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-
div.MenuBar ul li:hover ul.DropDownMenu li ul.SideMenu,
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div.MenuBar ul li a:hover ul.DropDownMenu li a ul.SideMenu {
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  display: none; /* Hides all side menus. */
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-
}
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-
/*------------------------------------------------------------------------------------- Menu Bar */
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-
div.MenuBar {
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  font: arial, helvetica, sans-serif;
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-
  height: 50px;
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-
  width: 910px;
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  /*width: 100%*/
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  margin: 0;
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  border-top: 0;
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  border-right: 0;
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  border-left: 0;
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  padding: 0;
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  background: black;
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-
}
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div.MenuBar ul {
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  font: arial, helvetica, sans-serif;
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-
  text-align: center;
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  list-style-type: none;
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  margin: 0;
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  border: 0;
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  padding: 0;
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  background: black;
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}
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div.MenuBar ul li {
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-
  font: arial, helvetica, sans-serif;
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-
  display: block;
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  padding: 0;
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  margin: 0;
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  font-size: 1.3em;
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  float: left;
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  background: black;
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  text-align: center;
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  width: 90px;
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  position: relative; /* Sets the positioning context for each drop-down menu. */
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}
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div.MenuBar ul li a {
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  font: arial, helvetica, sans-serif;
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-
  display: block;
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-
  background: black;
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-
  height: 40px; /* Keep height + padding-top + padding-bottom sync with the menu bar height. */
+
-
  color: #ffffff;
+
-
  padding-top: 4px;
+
-
  padding-bottom: 4px;
+
-
  padding-left: 1em; /* Sets the left space between top-level items. */
+
-
  padding-right: 1em; /* Sets the right space between top-level items. */
+
-
  text-decoration: none;
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-
}
+
-
 
+
-
/*------------------------------------------------------------------------------ Drop-Down Menus */
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-
div.MenuBar ul li:hover ul.DropDownMenu,
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-
div.MenuBar ul li a:hover ul.DropDownMenu {
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-
  display: block;
+
-
  width: 10em; /* Drop-down menu width.
+
-
                  Use MenuTailor.css to customize. */
+
-
  height: 1em;
+
-
  padding: 1px; /* Sets the drop-down menu "effective border" width. */
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-
  position: absolute;
+
-
  top: 23px; /* Places the drop-down menu under the menu bar.
+
-
                Keep it sync with the menu bar height. */
+
-
  left: 0; /* Aligns the drop-down menu to its top-level item. */
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-
  background-color: black; /* Selected item. */
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-
  color: #FFFFFF;
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-
 
+
-
}
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-
div.MenuBar ul li:hover ul.DropDownMenu li a,
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-
div.MenuBar ul li a:hover ul.DropDownMenu li a {
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-
  width: 10em; /* Keep it sync with the drop-down menu width.
+
-
                            Use MenuTailor.css to customize. */
+
-
  height: 1em;
+
-
  padding-left: 0;
+
-
  padding-right: 0;
+
-
  background-color: black; /* Selected item. */
+
-
  color: #FFFFFF;
+
-
}
+
-
ul.DropDownMenu li a span {
+
-
  display: block;
+
-
  padding-left: 0.75em; /* Sets the left space of each drop-down menu item. */
+
-
  padding-right: 0.25em; /* Sets the right space of each drop-down menu item. */
+
-
  text-align: right; /* Aligns the >> symbol to the right. */
+
-
}
+
-
ul.DropDownMenu li a span span {
+
-
  float: left; /* Aligns the text (back) to the left. */
+
-
  font: 12px arial, helvetica, sans-serif; /* Required for IE55. */
+
-
  height: 20px;
+
-
  color: #FFFFFF;
+
-
}
+
-
/*----------------------------------------------------------------------------------- Side Menus */
+
-
div.MenuBar ul li:hover ul.DropDownMenu li:hover ul.SideMenu,
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-
div.MenuBar ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu {
+
-
  display: block;
+
-
  width: 11em; /* Side menu width.
+
-
                  Use MenuTailor.css to customize. */
+
-
  padding: 1px; /* Sets the side menu "effective border" width. */
+
-
  position: absolute;
+
-
  top: -1px; /* Aligns the side menu to its drop-down menu item.
+
-
                Keep it sync with the side menu "effective border" width. */
+
-
  left: 13em; /* Places the side menu to the right of the drop-down menu.
+
-
                            Keep it sync with the drop-down menu width.
+
-
                            Use MenuTailor.css to customize. */
+
-
}
+
-
div.MenuBar ul li:hover ul.DropDownMenu li:hover ul.SideMenu li a,
+
-
div.MenuBar ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu li a {
+
-
  width: 11em; /* Keep it sync with the side menu width.
+
-
                            Use MenuTailor.css to customize. */
+
-
  font: 12px arial, helvetica, sans-serif; /* Required for IE55. */
+
-
  left: 13em; /* Places the side menu to the right of the drop-down menu.
+
-
                            Keep it sync with the drop-down menu width.
+
-
                            Use MenuTailor.css to customize. */
+
-
}
+
-
div.MenuBar ul li ul.DropDownMenu li ul.SideMenu li a span {
+
-
  padding-left: 1.5em; /* Sets the left space of each side menu item. */
+
-
  padding-right: 0.5em; /* Sets the right space of each side menu item. */
+
-
  text-align: left;
+
-
  font: 12px arial, helvetica, sans-serif; /* Required for IE55. */
+
-
  left: 13em; /* Places the side menu to the right of the drop-down menu.
+
-
                            Keep it sync with the drop-down menu width.
+
-
                            Use MenuTailor.css to customize. */
+
-
}
+
-
/*----------------------------------------------------------------------------- Browser Specific */
+
-
* html div.MenuBar ul li a {
+
-
  float: left; /* Required for IE55 and IE6.
+
-
                            Breaks O9.
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-
                            Hidden (* html) from non-IE browsers. */
+
-
}
+
-
* html ul.DropDownMenu li a:hover {
+
-
  cursor: hand; /* Required for IE55.
+
-
                  Hidden (* html) from non-IE browsers. */
+
-
}
+
-
ul.DropDownMenu li a:hover {
+
-
  cursor: pointer; /* Required for IE6 and IE7.
+
-
                      Hidding it (* html) from non-IE browsers breaks IE7!
+
-
}
+
-
* html div.MenuBar a:hover {
+
-
  text-decoration: none; /* Required for IE55 and IE6.
+
-
                            Hidden (* html) from non-IE browsers. */
+
-
}
+
-
* html div.MenuBar ul li table,
+
-
* html div.MenuBar ul li table td {
+
-
  border: 0; /* Required for IE55 and IE6.
+
-
                Hidden (* html) from non-IE browsers. */
+
-
}
+
-
/*------------------------------------------------------------------------------- Default Colors */
+
-
div.MenuBar {
+
-
  background-color: Menu;
+
-
  border-bottom: 1px solid ButtonShadow;
+
-
}
+
-
div.MenuBar a {
+
-
  background-color: Menu; /* Top-level unselected items. */
+
-
  color: MenuText;
+
-
}
+
-
div.MenuBar ul li:hover a,
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-
div.MenuBar ul li a:hover {
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-
  color: #ea7f16;
+
-
  background-color: Highlight; /* Top-level selected item. */
+
-
  color: HighlightText;
+
-
}
+
-
/*...............................................................................................*/
+
-
div.MenuBar ul li:hover ul.DropDownMenu,
+
-
div.MenuBar ul li a:hover ul.DropDownMenu {
+
-
  background-color: ButtonShadow; /* Sets the drop-down menu "effective border" color. */
+
-
}
+
-
div.MenuBar ul li:hover ul.DropDownMenu li a,
+
-
div.MenuBar ul li a:hover ul.DropDownMenu li a {
+
-
  background-color: Menu;  Drop-down menu unselected items.
+
-
                            Sets the drop-down menu "effective background" color. */
+
-
  color: MenuText;
+
-
}
+
-
div.MenuBar ul li:hover ul.DropDownMenu li:hover a,
+
-
div.MenuBar ul li a:hover ul.DropDownMenu li a:hover {
+
-
  background-color: Highlight; /* Drop-down menu selected item. */
+
-
  color: HighlightText;
+
-
}
+
-
/*...............................................................................................*/
+
-
div.MenuBar ul li:hover ul.DropDownMenu li:hover ul.SideMenu,
+
-
div.MenuBar ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu {
+
-
  background-color: ButtonShadow; /* Sets the side menu "effective border" color. */
+
-
}
+
-
div.MenuBar ul li:hover ul.DropDownMenu li:hover ul.SideMenu li a,
+
-
div.MenuBar ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu li a {
+
-
  background-color: Menu; /* Side menu unselected items.
+
-
                            Sets the side menu "effective background" color. */
+
-
  color: MenuText;
+
-
}
+
-
div.MenuBar ul li:hover ul.DropDownMenu li:hover ul.SideMenu li a:hover,
+
-
div.MenuBar ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu li a:hover {
+
-
  background-color: Highlight; /* Side menu selected item. */
+
-
  color: HighlightText;
+
-
}
+
-
/*-----------------------------------------------------------------------------------------------*/
+
-
/*Script-Free 3-Level Menu 1.2 Tailor
+
-
  www.CesarDaniel.info
+
-
/*-------------------------------------------------------------------------------------- General */
+
-
body {
+
-
  background: white;
+
-
  color: black;
+
-
  margin: 0;
+
-
  border: 0;
+
-
  padding: 0;
+
-
}
+
-
 
+
-
 
+
-
div.MenuBar {
+
-
  font: 13px arial, helvetica, sans-serif;
+
-
}
+
-
div.MenuBar ul {
+
-
  font: 13px arial, helvetica, sans-serif; /* Required for IE55. */
+
-
}
+
-
/*--------------------------------------------------------------------------------------- Colors */
+
-
div.MenuBar {
+
-
  background-color: black; /* Selected item. */
+
-
  color: #FFFFFF;
+
-
  border-bottom: 1px solid ButtonShadow;
+
-
}
+
-
div.MenuBar a,
+
-
div.MenuBar ul li:hover ul.DropDownMenu li a,
+
-
div.MenuBar ul li a:hover ul.DropDownMenu li a,
+
-
div.MenuBar ul li:hover ul.DropDownMenu li:hover ul.SideMenu li a,
+
-
div.MenuBar ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu li a {
+
-
  background-color: black; /* Selected item. */
+
-
  color: #FFFFFF;
+
-
}
+
-
div.MenuBar ul li:hover a,
+
-
div.MenuBar ul li a:hover,
+
-
div.MenuBar ul li:hover ul.DropDownMenu li:hover a,
+
-
div.MenuBar ul li a:hover ul.DropDownMenu li a:hover,
+
-
div.MenuBar ul li:hover ul.DropDownMenu li:hover ul.SideMenu li a:hover,
+
-
div.MenuBar ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu li a:hover {
+
-
  background-color: #603371; /* Selected item. */
+
-
  color: #FFFFFF;
+
-
}
+
-
div.MenuBar ul li:hover ul.DropDownMenu,
+
-
div.MenuBar ul li a:hover ul.DropDownMenu,
+
-
div.MenuBar ul li:hover ul.DropDownMenu li:hover ul.SideMenu,
+
-
div.MenuBar ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu {
+
-
  background-color: ButtonShadow; /* Sets the drop-down and side menus "effective border" color. */
+
-
}
+
-
/*--------------------------------------------------------------------------------------- Widths */
+
-
/*
+
-
 
+
-
/*
+
-
Menu Bar 1
+
-
Drop-Down Menu #2
+
-
*/
+
-
div.MenuBar#navi ul li:hover ul.DropDownMenu#MB1-DDM4,
+
-
div.MenuBar#navi ul li a:hover ul.DropDownMenu#MB1-DDM4,
+
-
div.MenuBar#navi ul li:hover ul.DropDownMenu#MB1-DDM4 li a,
+
-
div.MenuBar#navi ul li a:hover ul.DropDownMenu#MB1-DDM4 li a {
+
-
  width: 11em; /* Drop-down menu width. */
+
-
}
+
-
div.MenuBar#navi ul li:hover ul.DropDownMenu#MB1-DDM5,
+
-
div.MenuBar#navi ul li a:hover ul.DropDownMenu#MB1-DDM5,
+
-
div.MenuBar#navi ul li:hover ul.DropDownMenu#MB1-DDM5 li a,
+
-
div.MenuBar#navi ul li a:hover ul.DropDownMenu#MB1-DDM5 li a {
+
-
  width: 12em; /* Drop-down menu width. */
+
-
}
+
-
 
+
-
/*...............................................................................................*/
+
-
/*
+
-
Menu Bar 1
+
-
Drop-Down Menu #2
+
-
Side Menu #1
+
-
*/
+
-
div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM1,
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Inspired by the circadian clock in humans which regulates a number of very important processes, we are trying to replicate this biological clock in a bacterial system. We are aiming to construct a network of genes that oscillates in a similar fashion to the 24 hour system in humans. If we are successful, we will be able to put different genes into our system so that we can make the bacteria perform a particular process periodically – a simple example of this would be to make them flash on and off consistently.
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|[[File:UQ-Australia_logo_2011.png|125x125px|link=https://2011.igem.org/Team:UQ-Australia]]
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To achieve this oscillatory behaviour we will utilise a gene network with a series of inducible promoters that generate the production of other activating proteins, all driven by a constitutively active promoter. This promoter features an engineered repression domain (the inhibitor of this promoter being the output of the final step in the network). If everything goes as planned, these linked activations and repression will produce fluctuating levels of the proteins in question, which could then be used to drive our output function (initially just GFP production and a timed fluorescence). Ultimately, we hope our system could be used to drive the timed release of drugs or other biological factors.
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|align="center"|[[Team:UQ-Australia | Team Example]]
 
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Revision as of 04:33, 17 September 2011




You can write a background of your team here. Give us a background of your team, the members, etc. Or tell us more about something of your choosing. IGEM basic Logo stylized.png

Tell us more about your project. Give us background. Use this is the abstract of your project. Be descriptive but concise (1-2 paragraphs)

UQ-Australia logo 2011.png




Safety

1. Would any of your project ideas raise safety issues in terms of researcher, public, and environmental safety?

Any expected safety issues regarding researchers have been addressed prior to beginning the experimental work for iGEM. Members attended a series of inductions including Physical Containment 2 [http://www.ogtr.gov.au/internet/ogtr/publishing.nsf/content/PC2-4/$FILE/PC2LABv3-1-1.pdf (PC2)] training, Occupational Health & Safety [http://www.uq.edu.au/ohs/ (OHS)], and separate inductions for each lab by respective lab managers. Undergoing [http://www.ogtr.gov.au/internet/ogtr/publishing.nsf/content/PC2-4/$FILE/PC2LABv3-1-1.pdf PC2] training allows us to work with Genetically Modified materials inside a [http://www.ogtr.gov.au/internet/ogtr/publishing.nsf/content/PC2-4/$FILE/PC2LABv3-1-1.pdf PC2] lab, required for working with genetically modified organisms. Specific laboratory training was recorded in the Office of the Gene Technology Regulator [http://www.ogtr.gov.au/ (OGTR)] folder. This training was in conjunction to standard university online general induction training, annual fire safety training, and lab safety training. Student members were supervised in a lab environment when carrying out lab procedures or using lab equipments that required supervision, all with appropriate personal protective equipment (PPE) such as lab coat, gloves, closed shoes, and safety goggles; as indicated by material safety data sheets (MSDS) evaluated in risk assessments.

Any expected safety issues regarding public safety, have also been addressed prior to commencement of experimental work. To ensure the safety of the general public, no materials within the lab are exposed to the public or the environment. Transporting materials from one lab to another was done with the material in a double-sealed container. No personnel without [http://www.ogtr.gov.au/internet/ogtr/publishing.nsf/content/PC2-4/$FILE/PC2LABv3-1-1.pdf PC2] training were allowed in the lab. When disposing waste materials, biohazardous waste materials were disposed in the appropriate biohazard bin, likewise with sharps to ensure that general staff were not handling contaminated materials. Should an incident occur where others are placed in danger, an incident report is filed and handled by relevant personnel.

Any expected issues regarding environmental quality, have also been addressed prior to commencement experimental work. To ensure environmental safety, Environmental Management System [http://www.uq.edu.au/sustainability/policies-and-procedures (EMS)] induction was carried out, together with [http://www.ogtr.gov.au/internet/ogtr/publishing.nsf/content/PC2-4/$FILE/PC2LABv3-1-1.pdf PC2] training. Imported goods are required to go through the Australian Quarantine and Inspection Service [http://www.daff.gov.au/aqis (AQIS)]. They have rules regulating what is allowed into the country to ensure that the environment is not harmed. Therefore, any biological materials that we received from overseas or planning to send to overseas (for our project) is abides by the rules of [http://www.daff.gov.au/aqis AQIS].

The security of the materials was maintained in the [http://www.ogtr.gov.au/internet/ogtr/publishing.nsf/content/PC2-4/$FILE/PC2LABv3-1-1.pdf PC2] laboratory. The containment in the laboratory prevents untrained personnel from accessing materials. If we were to handle any Security Sensitive Biological Agents [http://www.health.gov.au/SSBA (SSBAs)], we would first consult the Biosafety Advisors at the University of Queensland.

2. Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues?

We anticipate that issues could be raised when transporting biological materials between Australia and Overseas. Imported goods are required to go through [http://www.daff.gov.au/aqis AQIS] who regulate what is allowed into the country to ensure that the environment is not harmed. Documentation will be submitted to the Registry regarding the transportation of biological materials to and from Australia.

3. Is there a local biosafety group, committee, or review board at your institution?

The University of Queensland has an Institutional Biosafety Committee [http://www.uq.edu.au/ohs/index.html?page=133431 (IBC)] who ensures that the university remains an accredited organisation under the [http://www.comlaw.gov.au/Details/C2011C00539 Gene Technology Act] and complies with the [http://www.legislation.qld.gov.au/LEGISLTN/CURRENT/W/WorkplHSaA95.pdf Queensland Workplace Health and Safety Act], Australian Standard 2243.3 Safety In Laboratories: Microbiological Safety and Containment, [http://www.daff.gov.au/aqis AQIS] and [http://www.ogtr.gov.au/ OGTR].

The [http://www.uq.edu.au/sustainability/policies-and-procedures EMS] is implemented by [http://www.uq.edu.au/sustainability/index.html Sustainability Office] at the University of Queensland ensures that all university personnel are familiar with environmental management relevant to them.

There is an [http://www.uq.edu.au/ohs/ OHS] committee at the School of Biomedical Sciences (where we are based) to ensure the safety of the researchers, public, and the environment. This is comprised of Workplace Health and Safety officers (WHSOs), [http://www.daff.gov.au/aqis AQIS] representatives, Workplace Health and Safety Representatives (WHSR), Biosafety Advisors, and Work Injury Management Team.

A discussion with the WHSOs resulted in the training of students. The students received [http://www.ogtr.gov.au/internet/ogtr/publishing.nsf/content/PC2-4/$FILE/PC2LABv3-1-1.pdf PC2] training, [http://www.uq.edu.au/ohs/ OHS] and [http://www.uq.edu.au/sustainability/policies-and-procedures EMS] induction and completed the online general safety, fire and laboratory training as well as laboratory specific training from the lab manager.

In Australia, the [http://www.comlaw.gov.au/Details/C2011C00539 Gene Technology Act] is national set of guidelines regulated by the [http://www.ogtr.gov.au/ OGTR]. [http://www.daff.gov.au/aqis AQIS] regulates the transportation of materials in and out of the country.

4. Do you have any other ideas how to deal with safety issues that could be useful for future iGEM competitions? How could parts, devices and systems be made even safer through biosafety engineering?

Current safety regulations have thus far been sufficient in maintaining the safety of the researchers, public and environment. Australia has its own set of regulations. We have informed iGEM of these regulations specific to Australia and anticipate that this information would update the iGEM safety information accessible to other teams.

Retrieved from "http://2011.igem.org/Team:UQ-Australia/Safety"