Team:Tokyo Metropolitan/Project

From 2011.igem.org

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== '''Overall project''' ==
== '''Overall project''' ==
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We would like to propose a brand new treatment using ''Escherichia coli''. We will make ''E.coli'' that moves in high speed to the target and kill it. We have been trying to put three different facility such as "speed up", "targeting", and "killing" by enhancing the flagellar movement, taking control of flagellar rotation, and sending killer gene to the target.
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We propose the project "Bee.coli" as the micro injection system. <br />
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Bee.coli has the ability to move fast and target the pathogens concentrated area. <br />
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Getting close to the pathogens, it sends the killer gene to them with conjugation. Then the pathogens will be killed.<br />
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To accomplish this project, we planed to add three function to E.coli.<br />
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First, expression of the mutant HNS proteins enable E.coli to move 1.5 times faster. This contributes to increasing the frequency of conjugation.<br />
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Second function is new taxis for AHL. We make the target bacteria as the model of the pathogen, and they produce AHL.
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Bee.coli will often go for where high concentration of AHL. That is, where the target bacteria are concentrated. <br />
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Last, the killer gene with the conjugative plasmid. Since Bee.coli also has the anti killer gene, it is alive and kill the pathogens with conjugation.<br />
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In the future, Bee.coli will help the medical or environmental scenes.
== Project Details==
== Project Details==

Revision as of 15:54, 1 September 2011

Contents

Overall project

We propose the project "Bee.coli" as the micro injection system.
Bee.coli has the ability to move fast and target the pathogens concentrated area.
Getting close to the pathogens, it sends the killer gene to them with conjugation. Then the pathogens will be killed.
To accomplish this project, we planed to add three function to E.coli.
First, expression of the mutant HNS proteins enable E.coli to move 1.5 times faster. This contributes to increasing the frequency of conjugation.
Second function is new taxis for AHL. We make the target bacteria as the model of the pathogen, and they produce AHL. Bee.coli will often go for where high concentration of AHL. That is, where the target bacteria are concentrated.
Last, the killer gene with the conjugative plasmid. Since Bee.coli also has the anti killer gene, it is alive and kill the pathogens with conjugation.
In the future, Bee.coli will help the medical or environmental scenes.

Project Details

Part 2

The Experiments

Part 3

Results

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