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Team:Freiburg/Notebook/30 August
From 2011.igem.org
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==<span style="color:green;">green light receptor</span>== | ==<span style="color:green;">green light receptor</span>== | ||
| - | === | + | ===Ligation of Promotor PcpcG in PSB1C3=== |
| - | + | ||
| - | + | ||
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| + | '''Investigators:Julia''' | ||
| + | <br/> | ||
| + | <br/> | ||
| + | 1. Digestion of PCR Product from yesterday[https://2011.igem.org/Team:Freiburg/Notebook/29_August]<br/> | ||
| + | <br/> | ||
| + | 38µl PCR product<br/> | ||
| + | 5µl BSA (10x)<br/> | ||
| + | 5µl NEB buffer 4<br/> | ||
| + | 1µl EcoRI<br/> | ||
| + | 1µlPstI<br/> | ||
| + | <br/> | ||
| + | Incubation for 3 hours, afterwards the sample was cleaned with a PCR purification kit from Qiagen.<br/> | ||
| + | <br/> | ||
| + | 2.Ligation<br/> | ||
| + | <br/>11µl H2O<br/>4µl PcpcG<br/>2µl pSB1C3<br/>2µl T4 Ligase buffer<br/> 1µl T4 Ligase<br/> | ||
==<span style="color:blue;">blue light receptor</span>== | ==<span style="color:blue;">blue light receptor</span>== | ||
Revision as of 19:16, 30 August 2011
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This is the wiki page of the Freiburger student team competing for iGEM 2011. Thank you for your interest!
