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Todo

Microfluidic Chips

• Continue alignment training

Preparing the parts

We need to sequence-verify the lysis brick and LacI.

Status: Primers for sequencing the lysis brick have been ordered; reaction solutions for LacI sequencing have been prepared (ready to send out?).

Next steps:

• Make competent cells
• Test competent cells ~10^5CFU/µg not very good
• Transform in E. Coli.
• Glycerol Stocks
• Plasmid preps
• Sequence the lysis cassette

Assembly

• Research plasmid backbones. Is there one that already contains Tet-repressed LacI or GFP?
• Design Gibson primers to assemble the three different plasmids.
• Determine which sequence on LacI-plasmid and Lysis-plasmid should be used to create the "mega-plasmid".

Wiki

For anybody:

• Start uploading protocols
• Write-up team presentation

For an HTML-literate person:

• Activate Javascript accordion menu
• Fix alignement
• Fix titles

Clean rooms

• Order lab notebook
• Order storage box