Team:Freiburg/Notebook/29 August

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*♥-T3 nOt
*♥-T3 nOt
*♥-T3 noT
*♥-T3 noT
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Incubation over night at 37°C.
==<span style="color:red;">red light receptor</span>==
==<span style="color:red;">red light receptor</span>==

Revision as of 15:51, 29 August 2011


This is the wiki page
of the Freiburger student
team competing for iGEM 2011.
Thank you for your interest!

Contents

green light receptor

PCR to amplify Green light Promotor PcpcG

Investigators:Julia

Testdigest of quickchanged CcaR

Investigators:Julia,with help of the team

blue light receptor

3A-assembly

Investigators: Sandra

Digestion I:

  • Lovtap
  • Not-Gate
  • pSB1A3
  • pSB1T3

stored in the blue light box.

Ligation

  • ♥-A3 Not
  • ♥-A3 nOt
  • ♥-A3 noT
  • ♥-T3 Not
  • ♥-T3 nOt
  • ♥-T3 noT

stored in the ligation box.

Digestion II:

Also digestion with new amplified vector

  • pSB1C3

Lovtap was digested again this time also with DpnI. So for this ligation the ♥-PCR3A, digested with DpnI, and pSB1C3 (new amplified with PCR, already digested with DpnI) and Not, nOt, noT were used.

  • ♥-C3 Not
  • ♥-C3 nOt
  • ♥-C3 noT

Transformation

Investigators: Sophie

transformation of:

  • ♥-A3 Not
  • ♥-A3 nOt
  • ♥-A3 noT
  • ♥-T3 Not
  • ♥-T3 nOt
  • ♥-T3 noT

Incubation over night at 37°C.

red light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME



Lysis cassette

NAME OF YOUR EXPERIMENT

Investigators:NAME


Precipitator

NAME OF YOUR EXPERIMENT

Investigators: NAME

                       

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