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Team:Bielefeld-Germany/HPLC isocratic
From 2011.igem.org
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** Series 1050 by [http://www.hp.com/ Hewlett Packard] | ** Series 1050 by [http://www.hp.com/ Hewlett Packard] | ||
| - | [[File:Bielefeld2011 BPA + Abbauprodukt-2.JPG|900px]] | + | [[File:Bielefeld2011 BPA + Abbauprodukt-2.JPG|900px|Chromatogram obtained using the above described materials and methods. Three distinctive Peak are of interest: BPA at 7,3 min retention time, BPF at 5,8 min retention time and ]] |
[[File:Bielefeld2011 BPA + Abbauprodukt-3.JPG|900px]] | [[File:Bielefeld2011 BPA + Abbauprodukt-3.JPG|900px]] | ||
[[File:Bielefeld2011 BPA + Abbauprodukt.JPG|900px]] | [[File:Bielefeld2011 BPA + Abbauprodukt.JPG|900px]] | ||
Revision as of 19:33, 29 August 2011
HPLC method
- C18 reverse phase column
- Isocratic method: 45 % acetonitrile
- Flow = 0.6 mL min-1
- UV-detection at 227 nm
- Column:
- Eurospher II 100-5 C18p by [http://www.knauer.net/ Knauer]
- Dimensions: 150 x 4.6 mm with precolumn
- Particle size: 5 µm
- Pore size: 100 Å
- Material: silica gel
- Software:
- Clarity (Version 3.0.5.505) by [http://www.dataapex.com/ Data Apex]
- Autosampler:
- Midas by [http://www.spark.nl/ Spark Holland]
- Tray cooling: 10 °C
- Pump:
- L-6200A Intelligent Pump by [http://www.hitachi.com/ Hitachi]
- UV-Detector:
- Series 1050 by [http://www.hp.com/ Hewlett Packard]
