Team:Sevilla/Week4

From 2011.igem.org

(Difference between revisions)
Line 162: Line 162:
<p>
<p>
-
The primers we ordered last week should have come today, but as usual, they haven't (bureaucracy, lazy postmen, maybe a natural catastrophe...). This means we cannot do much today, except for planning our next steps, searching for more information about how to build logic gates or deciphering the intrincate registry of parts. We've realised we have some useful biobricks in our distribution kit we didn't even know about.
+
The primers we ordered last week should have come today, but as usual, they haven't (bureaucracy, lazy postmen, maybe a natural catastrophe...). This means we can't do much today, except plan our next steps and search for more information about how to build logic gates, or decipher the intrincate registry of parts. We've realised we have some useful biobricks in our distribution kit which we didn't even know about.
</p>
</p>
-
<p>Another useful thing to spend time: preparing the videos for the people who gave us money through the crowdfunding platform, in appreciation for their donations.
+
 
 +
<p>Another useful way of spending time: preparing the videos for the people who gave us money through the crowdfunding platform, in appreciation for their donations.
</p>
</p>
<p>
<p>
-
Even more useful: piling the pipette's tips to build a giant ring!
+
Even more useful: joining together pipette tips to build a giant ring!
</p>
</p>
Line 206: Line 207:
<p>
<p>
-
Our primers still don't arrive, but to ease our boredom, the university's cabling decides to short-circuit! Our incubator starts steaming, the fire alarm starts ringing, the fridges don't work, our frozen cultures start to melt, but we don't panic...until we find out the Internet doesn't work either!!!
+
Our primers still haven't arrived, but happily, to ease our boredom the university's cabling
-
 
+
decides to short-circuit! Our incubator starts steaming, the fire alarm starts ringing, the fridges
 +
don't work, our frozen cultures start to melt, but we don't panic...until we find out the Internet
 +
doesn't work either!!!
</p>
</p>
-
 
<img src="https://lh6.googleusercontent.com/-_W5Zw0MToVQ/TjkvzOGMWCI/AAAAAAAAAgI/qEJHcFeSt2Y/s288/IMG_5117.JPG" style=padding-left:160px;"height="192" width="288" />
<img src="https://lh6.googleusercontent.com/-_W5Zw0MToVQ/TjkvzOGMWCI/AAAAAAAAAgI/qEJHcFeSt2Y/s288/IMG_5117.JPG" style=padding-left:160px;"height="192" width="288" />
Line 244: Line 246:
<p>
<p>
-
 
+
More mini-preps, more transformations, and more competent cells to kill the time while the
-
More mini-preps, transformations, competent cells to kill the time while the primers arrive, but at least we're improving and the results are pretty good! Besides, we're trying a new protocol to purify DNA after digestions. Tomorrow we'll see how it goes.
+
primers arrive, but at least we're improving and the results are pretty good! Besides, we're
-
 
+
trying out a new protocol to purify DNA after digestions. Tomorrow we'll see how it goes.
</p>
</p>
Line 282: Line 284:
<p>
<p>
-
After purifications we run an electrophoresis and it turns out to be so beautiful we almost cry! Everything has gone as expected, the digestions are ok, but the NanoDrop results say we've lost too much DNA in the process. </p>
+
After purifications we run an electrophoresis and it turns out so beautifully we almost
-
 
+
cry! Everything has gone as expected, the digestions are ok, but the NanoDrop results
 +
say we've lost too much DNA in the process.</p>
<p>
<p>
-
The primers are here at last! We can start with the pcr reactions to regenerate plasmid backbones and obtain some genes from our donated strains.Let's see how we go...
+
The primers are here at last! We can finally begin the PCR reactions to regenerate
 +
plasmid backbones and obtain some genes from our donated strains. Let's see how we
 +
go...
 +
 
</p>
</p>
 +
<img src="https://lh3.googleusercontent.com/-2IlQLrH-_E8/Tju2g0_kTtI/AAAAAAAAAmk/_K-tv6TOEF8/s288/IMG_5204.JPG" style=padding-left:200px;"height="192" width="288" />
<img src="https://lh3.googleusercontent.com/-2IlQLrH-_E8/Tju2g0_kTtI/AAAAAAAAAmk/_K-tv6TOEF8/s288/IMG_5204.JPG" style=padding-left:200px;"height="192" width="288" />
Line 322: Line 329:
<p>
<p>
-
Roberto has brought m&m's, lots of them!!!This is the best day of our lives. Besides, the PCR results are quite good,e xcept for the fact that we've amplified the wrong BioBrick - it seems working here for so many hours has overheat our brains. We'll have to try next week, paying more attention.
+
Roberto has brought M&M's: lots of them!!!This is the best day of our lives. Besides, the PCR
 +
results are really quite good, except for the fact that we've amplified the wrong BioBrick - it
 +
seems working here for so many hours has overheated our brains. We'll have to try again next
 +
week, and make sure we pay more attention.
 +
 
</p>
</p>
Line 358: Line 369:
<div class="summary">
<div class="summary">
-
<p>We have a bit of a do to celebrate Roberto's birthday. Look how elegant we can be in special ocasions!
+
<p>We have a bit of a do to celebrate Roberto's birthday. Look how elegant we can be on special occasions!
</p>
</p>
<img src="https://lh5.googleusercontent.com/-hPzzVSFmuX4/Tj-sSlBbAqI/AAAAAAAAAvM/wSJLBnTY3jE/s400/IMG_5332.JPG" style=padding-left:200px;"height="267" width="400" />
<img src="https://lh5.googleusercontent.com/-hPzzVSFmuX4/Tj-sSlBbAqI/AAAAAAAAAvM/wSJLBnTY3jE/s400/IMG_5332.JPG" style=padding-left:200px;"height="267" width="400" />
-
<p>We also spend the whole sunday in a "Game of Thrones" marathon at Marta's eating pizza. What an awsome series! Highly recommended by Arcanum,you can't miss it!</p>
+
<p>We also spend the whole Sunday watching a "Game of Thrones" marathon at Marta's while eating pizza. What an awsome series! Highly recommended by Arcanum - you can't miss it!</p>
<input type="button" value="Show weekend protocols" id="weekendBP">
<input type="button" value="Show weekend protocols" id="weekendBP">
-
 
</div>
</div>

Revision as of 05:56, 19 September 2011


Monday 1 August


The primers we ordered last week should have come today, but as usual, they haven't (bureaucracy, lazy postmen, maybe a natural catastrophe...). This means we can't do much today, except plan our next steps and search for more information about how to build logic gates, or decipher the intrincate registry of parts. We've realised we have some useful biobricks in our distribution kit which we didn't even know about.

Another useful way of spending time: preparing the videos for the people who gave us money through the crowdfunding platform, in appreciation for their donations.

Even more useful: joining together pipette tips to build a giant ring!


Monday Protocols


In. Mid vel vel elementum, placerat tincidunt, vut lacus purus nunc massa a habitasse? Ac pid elementum aliquam, natoque! Porttitor facilisis amet lacus, ridiculus nascetur! Sociis vel aenean natoque penatibus aliquam in, augue mauris, dictumst, nunc mid platea a! Massa? Vut dis purus. Odio magna nec. Lorem ut turpis adipiscing scelerisque dis, scelerisque. Sed quis, porttitor et pulvinar in. Massa sit, porta urna nunc auctor non elementum! Augue dolor cum sociis, dignissim elementum. Integer. Penatibus. Phasellus et porta nec! Hac in nec porttitor ridiculus rhoncus. Hac natoque mid est nunc platea nisi sed habitasse! Amet nec placerat ultricies egestas. Vel cum diam, lorem integer et montes, nec placerat, turpis lacus nec placerat, rhoncus rhoncus, a porta augue ac! Et aliquet. Porttitor diam egestas, non vut etiam montes, aliquet? Pellentesque. Dolor nascetur amet. Adipiscing sed pulvinar cursus! Urna in, in turpis duis in sit placerat, vut non aliquam? Mattis habitasse augue mus. Pulvinar auctor natoque est lacus porta sagittis! A in massa lundium? In non, lundium nisi magna proin elementum sed porttitor sed amet risus a aliquet mid a? Proin turpis, ac magna, dictumst lorem tincidunt penatibus sociis rhoncus? Duis cursus sit diam amet nisi mattis egestas habitasse diam.


Tuesday 2 August


Our primers still haven't arrived, but happily, to ease our boredom the university's cabling decides to short-circuit! Our incubator starts steaming, the fire alarm starts ringing, the fridges don't work, our frozen cultures start to melt, but we don't panic...until we find out the Internet doesn't work either!!!


Tuesday Protocols


In. Mid vel vel elementum, placerat tincidunt, vut lacus purus nunc massa a habitasse? Ac pid elementum aliquam, natoque! Porttitor facilisis amet lacus, ridiculus nascetur! Sociis vel aenean natoque penatibus aliquam in, augue mauris, dictumst, nunc mid platea a! Massa? Vut dis purus. Odio magna nec. Lorem ut turpis adipiscing scelerisque dis, scelerisque. Sed quis, porttitor et pulvinar in. Massa sit, porta urna nunc auctor non elementum! Augue dolor cum sociis, dignissim elementum. Integer. Penatibus. Phasellus et porta nec! Hac in nec porttitor ridiculus rhoncus. Hac natoque mid est nunc platea nisi sed habitasse! Amet nec placerat ultricies egestas. Vel cum diam, lorem integer et montes, nec placerat, turpis lacus nec placerat, rhoncus rhoncus, a porta augue ac! Et aliquet. Porttitor diam egestas, non vut etiam montes, aliquet? Pellentesque. Dolor nascetur amet. Adipiscing sed pulvinar cursus! Urna in, in turpis duis in sit placerat, vut non aliquam? Mattis habitasse augue mus. Pulvinar auctor natoque est lacus porta sagittis! A in massa lundium? In non, lundium nisi magna proin elementum sed porttitor sed amet risus a aliquet mid a? Proin turpis, ac magna, dictumst lorem tincidunt penatibus sociis rhoncus? Duis cursus sit diam amet nisi mattis egestas habitasse diam.


Wednesday 3 August


More mini-preps, more transformations, and more competent cells to kill the time while the primers arrive, but at least we're improving and the results are pretty good! Besides, we're trying out a new protocol to purify DNA after digestions. Tomorrow we'll see how it goes.


Wednesday Protocols


In. Mid vel vel elementum, placerat tincidunt, vut lacus purus nunc massa a habitasse? Ac pid elementum aliquam, natoque! Porttitor facilisis amet lacus, ridiculus nascetur! Sociis vel aenean natoque penatibus aliquam in, augue mauris, dictumst, nunc mid platea a! Massa? Vut dis purus. Odio magna nec. Lorem ut turpis adipiscing scelerisque dis, scelerisque. Sed quis, porttitor et pulvinar in. Massa sit, porta urna nunc auctor non elementum! Augue dolor cum sociis, dignissim elementum. Integer. Penatibus. Phasellus et porta nec! Hac in nec porttitor ridiculus rhoncus. Hac natoque mid est nunc platea nisi sed habitasse! Amet nec placerat ultricies egestas. Vel cum diam, lorem integer et montes, nec placerat, turpis lacus nec placerat, rhoncus rhoncus, a porta augue ac! Et aliquet. Porttitor diam egestas, non vut etiam montes, aliquet? Pellentesque. Dolor nascetur amet. Adipiscing sed pulvinar cursus! Urna in, in turpis duis in sit placerat, vut non aliquam? Mattis habitasse augue mus. Pulvinar auctor natoque est lacus porta sagittis! A in massa lundium? In non, lundium nisi magna proin elementum sed porttitor sed amet risus a aliquet mid a? Proin turpis, ac magna, dictumst lorem tincidunt penatibus sociis rhoncus? Duis cursus sit diam amet nisi mattis egestas habitasse diam.


Thursday 4 August


After purifications we run an electrophoresis and it turns out so beautifully we almost cry! Everything has gone as expected, the digestions are ok, but the NanoDrop results say we've lost too much DNA in the process.

The primers are here at last! We can finally begin the PCR reactions to regenerate plasmid backbones and obtain some genes from our donated strains. Let's see how we go...


Thursday Protocols


In. Mid vel vel elementum, placerat tincidunt, vut lacus purus nunc massa a habitasse? Ac pid elementum aliquam, natoque! Porttitor facilisis amet lacus, ridiculus nascetur! Sociis vel aenean natoque penatibus aliquam in, augue mauris, dictumst, nunc mid platea a! Massa? Vut dis purus. Odio magna nec. Lorem ut turpis adipiscing scelerisque dis, scelerisque. Sed quis, porttitor et pulvinar in. Massa sit, porta urna nunc auctor non elementum! Augue dolor cum sociis, dignissim elementum. Integer. Penatibus. Phasellus et porta nec! Hac in nec porttitor ridiculus rhoncus. Hac natoque mid est nunc platea nisi sed habitasse! Amet nec placerat ultricies egestas. Vel cum diam, lorem integer et montes, nec placerat, turpis lacus nec placerat, rhoncus rhoncus, a porta augue ac! Et aliquet. Porttitor diam egestas, non vut etiam montes, aliquet? Pellentesque. Dolor nascetur amet. Adipiscing sed pulvinar cursus! Urna in, in turpis duis in sit placerat, vut non aliquam? Mattis habitasse augue mus. Pulvinar auctor natoque est lacus porta sagittis! A in massa lundium? In non, lundium nisi magna proin elementum sed porttitor sed amet risus a aliquet mid a? Proin turpis, ac magna, dictumst lorem tincidunt penatibus sociis rhoncus? Duis cursus sit diam amet nisi mattis egestas habitasse diam.


Friday 5 August


Roberto has brought M&M's: lots of them!!!This is the best day of our lives. Besides, the PCR results are really quite good, except for the fact that we've amplified the wrong BioBrick - it seems working here for so many hours has overheated our brains. We'll have to try again next week, and make sure we pay more attention.


Friday Protocols


In. Mid vel vel elementum, placerat tincidunt, vut lacus purus nunc massa a habitasse? Ac pid elementum aliquam, natoque! Porttitor facilisis amet lacus, ridiculus nascetur! Sociis vel aenean natoque penatibus aliquam in, augue mauris, dictumst, nunc mid platea a! Massa? Vut dis purus. Odio magna nec. Lorem ut turpis adipiscing scelerisque dis, scelerisque. Sed quis, porttitor et pulvinar in. Massa sit, porta urna nunc auctor non elementum! Augue dolor cum sociis, dignissim elementum. Integer. Penatibus. Phasellus et porta nec! Hac in nec porttitor ridiculus rhoncus. Hac natoque mid est nunc platea nisi sed habitasse! Amet nec placerat ultricies egestas. Vel cum diam, lorem integer et montes, nec placerat, turpis lacus nec placerat, rhoncus rhoncus, a porta augue ac! Et aliquet. Porttitor diam egestas, non vut etiam montes, aliquet? Pellentesque. Dolor nascetur amet. Adipiscing sed pulvinar cursus! Urna in, in turpis duis in sit placerat, vut non aliquam? Mattis habitasse augue mus. Pulvinar auctor natoque est lacus porta sagittis! A in massa lundium? In non, lundium nisi magna proin elementum sed porttitor sed amet risus a aliquet mid a? Proin turpis, ac magna, dictumst lorem tincidunt penatibus sociis rhoncus? Duis cursus sit diam amet nisi mattis egestas habitasse diam.


Weekend


We have a bit of a do to celebrate Roberto's birthday. Look how elegant we can be on special occasions!

We also spend the whole Sunday watching a "Game of Thrones" marathon at Marta's while eating pizza. What an awsome series! Highly recommended by Arcanum - you can't miss it!


Weekend Protocols


In. Mid vel vel elementum, placerat tincidunt, vut lacus purus nunc massa a habitasse? Ac pid elementum aliquam, natoque! Porttitor facilisis amet lacus, ridiculus nascetur! Sociis vel aenean natoque penatibus aliquam in, augue mauris, dictumst, nunc mid platea a! Massa? Vut dis purus. Odio magna nec. Lorem ut turpis adipiscing scelerisque dis, scelerisque. Sed quis, porttitor et pulvinar in. Massa sit, porta urna nunc auctor non elementum! Augue dolor cum sociis, dignissim elementum. Integer. Penatibus. Phasellus et porta nec! Hac in nec porttitor ridiculus rhoncus. Hac natoque mid est nunc platea nisi sed habitasse! Amet nec placerat ultricies egestas. Vel cum diam, lorem integer et montes, nec placerat, turpis lacus nec placerat, rhoncus rhoncus, a porta augue ac! Et aliquet. Porttitor diam egestas, non vut etiam montes, aliquet? Pellentesque. Dolor nascetur amet. Adipiscing sed pulvinar cursus! Urna in, in turpis duis in sit placerat, vut non aliquam? Mattis habitasse augue mus. Pulvinar auctor natoque est lacus porta sagittis! A in massa lundium? In non, lundium nisi magna proin elementum sed porttitor sed amet risus a aliquet mid a? Proin turpis, ac magna, dictumst lorem tincidunt penatibus sociis rhoncus? Duis cursus sit diam amet nisi mattis egestas habitasse diam.

Retrieved from "http://2011.igem.org/Team:Sevilla/Week4"