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Team:Bielefeld-Germany/HPLC isocratic
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===HPLC method=== | ===HPLC method=== | ||
* C18 reverse phase column | * C18 reverse phase column | ||
| - | * Isocratic method: 45% [[Team:Bielefeld-Germany/Protocols#Used_chemicals | | + | * Isocratic method: 45% [[Team:Bielefeld-Germany/Protocols#Used_chemicals | acetonitrile]] |
* Flow = 0.6 mL/min | * Flow = 0.6 mL/min | ||
* UV-detection at 227 nm | * UV-detection at 227 nm | ||
Revision as of 09:22, 23 August 2011
HPLC method
- C18 reverse phase column
- Isocratic method: 45% acetonitrile
- Flow = 0.6 mL/min
- UV-detection at 227 nm
- Column:
- Eurospher II 100-5 C18p
- Dimensions: 150 x 4.6 mm with precolumn
- Particle size: 5 µm
- Pore size: 100 Å
- Material: silica gel
