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Team:UNICAMP-EMSE Brazil/protocols/Digestion
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| - | ==Digestion== | + | [[Image:UNICAMP-EMSE_Brazil_E_Coli.png|270px|right]] |
| + | ==<font size="5" color=#086A87>Digestion:== | ||
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(For each 1000 ng of DNA use 1 U of enzyme in 20uL reaction) | (For each 1000 ng of DNA use 1 U of enzyme in 20uL reaction) | ||
Revision as of 00:49, 23 August 2011
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Digestion:
(For each 1000 ng of DNA use 1 U of enzyme in 20uL reaction)
- Use 3000 ng BioBrick vector
- 6 µL 10x buffer
- 0.5 µL enzyme 1 (20 units/µL)
- 0.5 µL enzyme 2 (20 units/µL)
- distilled water to 60 µL total volume
Put in 0,2 mL eppendorf tubes and let 2 h at 37°C.
Make an electrophoresys (use the hole reaction volume) and cut the band with your gene of interest.
