Team:UNICAMP-EMSE Brazil/protocols/Digestion

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Make an electrophoresys (use the hole reaction volume) and cut the band with your gene of interest.
Make an electrophoresys (use the hole reaction volume) and cut the band with your gene of interest.
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Revision as of 00:27, 23 August 2011

Home Project Methods Results Data Team Notebook Human Practices Safety Profile Sponsors Wix

Digestion

(For each 1000 ng of DNA use 1 U of enzyme in 20uL reaction)

  1. Use 3000 ng BioBrick vector
  2. 6 µL 10x buffer
  3. 0.5 µL enzyme 1 (20 units/µL)
  4. 0.5 µL enzyme 2 (20 units/µL)
  5. distilled water to 60 µL total volume

Put in 0,2 mL eppendorf tubes and let 2 h at 37°C.

Make an electrophoresys (use the hole reaction volume) and cut the band with your gene of interest.






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