Team:UNICAMP-EMSE Brazil/Notebook/20 August 2011
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+ | {{Template:UNICAMP-EMSE Brazil_Notebook}} | ||
+ | <div style="text-align: left;"> | ||
+ | ==20 August 2011== | ||
+ | ===Everybody:=== | ||
+ | '''Objective:''' Check if the size of the synthetic DNA sequences that didn´t work on 19/08/2011. | ||
+ | *Agarose: 0,8% | ||
+ | *Ladder: 100-10.000 bp (Fermentas) | ||
+ | |||
+ | *'''Picture: Upper part:''' | ||
+ | #'''Gel 1:''' flHDC1 (nd)/ flHDC1 (d)/ flHDC2 (nd)/ flHDC2 (d)/RBSTolC3 (nd)/ RBSTolC3(d)/ RBSTolC4 (nd)/ RBSTolC4 (d)/RBSGFP1 (nd)/RBSGFP1 (D)/ RBSGFP 3(ND)/RBSGFP 3 (D)/ RBSQSEBRBSQSEC 3 (ND)/ RBSQSEBRBSQSEC 3 (D) | ||
+ | #'''Gel 2:''' / RBSQSEBRBSQSEC 4 (ND)/ RBSQSEBRBSQSEC 4 (D)/STRONG PROMOTOR 3 (ND)/ STRONG PROMOTOR 3 (D)/ STRONG PROMOTOR 4 (ND)/ STRONG PROMOTOR 4 (D)/ SOX S 3 (ND)/ SOX S 3 (D)/ SOX S 4 (ND)/SOX S 4 (D) | ||
+ | |||
+ | Nd: non digested | ||
+ | D: digested | ||
+ | |||
+ | |||
[[Image:UNICAMP-EMSE_Brazil_Gel_20_08_11.jpg|300px|thumb|right|Electrophoresis Gel, 20/08/2011]] | [[Image:UNICAMP-EMSE_Brazil_Gel_20_08_11.jpg|300px|thumb|right|Electrophoresis Gel, 20/08/2011]] | ||
+ | </div> |
Revision as of 20:23, 21 August 2011
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20 August 2011
Everybody:
Objective: Check if the size of the synthetic DNA sequences that didn´t work on 19/08/2011.
- Agarose: 0,8%
- Ladder: 100-10.000 bp (Fermentas)
- Picture: Upper part:
- Gel 1: flHDC1 (nd)/ flHDC1 (d)/ flHDC2 (nd)/ flHDC2 (d)/RBSTolC3 (nd)/ RBSTolC3(d)/ RBSTolC4 (nd)/ RBSTolC4 (d)/RBSGFP1 (nd)/RBSGFP1 (D)/ RBSGFP 3(ND)/RBSGFP 3 (D)/ RBSQSEBRBSQSEC 3 (ND)/ RBSQSEBRBSQSEC 3 (D)
- Gel 2: / RBSQSEBRBSQSEC 4 (ND)/ RBSQSEBRBSQSEC 4 (D)/STRONG PROMOTOR 3 (ND)/ STRONG PROMOTOR 3 (D)/ STRONG PROMOTOR 4 (ND)/ STRONG PROMOTOR 4 (D)/ SOX S 3 (ND)/ SOX S 3 (D)/ SOX S 4 (ND)/SOX S 4 (D)
Nd: non digested D: digested