Revision as of 16:14, 24 May 2011

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We need to sequence-verify the lysis brick and LacI.

Status: Primers for sequencing the lysis brick have been ordered; reaction solutions for LacI sequencing have been prepared (ready to send out?).

Next steps:

Research plasmid backbones. Two different types are needed.
Design Gibson primers to assemble the three different plasmids
Determine which sequence on plasmids "two" and "three" should be used to create the "mega-plasmid"

For anybody:

For an HTML-literate person:

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 Next steps:
 * <s>Make competent cells</s>
+* -> Test competent cells
 * Transform in E. Coli.
 * Glycerol Stocks