Team:Queens Canada/Notebook/Protocols/Rehydration

From 2011.igem.org

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<regulartext> <b> Materials </b> </regulartext> <br>
<regulartext> <b> Materials </b> </regulartext> <br>
<regulartext> - Dry primers </regulartext> <br>
<regulartext> - Dry primers </regulartext> <br>
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<regulartext> - ddH2O </regulartext> <br>
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<regulartext> - ddH2O </regulartext> <p>
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<regulartext> <b> Procedure </b> </regulartext> <br>
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<regulartext> 1. Ensure that you know what the primer that you are rehydrating is for so that you can label it properly.  </regulartext> <br>
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<regulartext> 2. Using sterile technique, add the correct volume of ddH2O to the dry primers such that they make a 100μM solution (consult the shipping invoice to see what the volume of water should be). If the volume specified by the invoice is over 900μL, add half the specified volume (to make a 200μM solution). Label the top of the tube with the concentration of the primer.    </regulartext> <br>
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Revision as of 12:39, 19 September 2011

Queen's
rehydration
Rehydration- iGEM Standard Parts

Storage and Labelling
- Rehydrate primers just before they are needed for PCR
- Store primers (both concentrations) in the -20C freezer

Materials
- Dry primers
- ddH2O

Procedure
1. Ensure that you know what the primer that you are rehydrating is for so that you can label it properly.
2. Using sterile technique, add the correct volume of ddH2O to the dry primers such that they make a 100μM solution (consult the shipping invoice to see what the volume of water should be). If the volume specified by the invoice is over 900μL, add half the specified volume (to make a 200μM solution). Label the top of the tube with the concentration of the primer.

Rehydration- Primers

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