Team:Freiburg/Notebook/8 August

From 2011.igem.org

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'''Investigators: Sophie'''<br/>
'''Investigators: Sophie'''<br/>
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continue from experiment: miniprep (the, 8.8.)
+
continue from experiment: miniprep (the, 8.8.)<br/>
 +
Project name: new 3A assembly with amp vector
 +
Parts: psB1A3 (Eco & Pst)<br/>
 +
GFP-pbd ( Xba & Pst)<br/>
 +
PR4 or PR6 (Eco & Spe)<br/>
 +
Why? The last cloning of Theo and Jakob produced one clone with GFP-pbd inside but without PR. So I'm trying again to clone with two different PRs with different RBS strenghts.<br/>
 +
Stored in: "Minipreps, verdaut"-box
 +
 
{{:Team:Freiburg/Templates/footer}}
{{:Team:Freiburg/Templates/footer}}

Revision as of 12:05, 16 August 2011


This is the wiki page
of the Freiburger student
team competing for iGEM 2011.
Thank you for your interest!

Contents

green light receptor

Quickchange


Investigator:Jakob

PCR


Name:

Jakob

Date:

08.08.2011

Continue from Experiment:
Project Name:

Quickchange CcaR and CcaS

PCR-Mixture for one Reaction:

For a 50 µl reaction use


32,5µl H20
10µl 5x Phusion Buffer
2.5µl Primer fw Primer: P12(CcaR) and P14(CcaS
2.5µl Primer dw Primer: P13(CcaR) and P15(CcaS)
1µl dNTPs
1µl DNA-Template DNA: CcaR and CcaS
0.5 µl Phusion (add in the end)



Digest with DpnI

Transformation

blue light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME



red light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME



Lysis cassette

NAME OF YOUR EXPERIMENT

Investigators:NAME


Precipitator

NAME OF YOUR EXPERIMENT: Cloning

Investigators: Sophie
continue from experiment: miniprep (the, 8.8.)
Project name: new 3A assembly with amp vector Parts: psB1A3 (Eco & Pst)

GFP-pbd ( Xba & Pst)

PR4 or PR6 (Eco & Spe)
Why? The last cloning of Theo and Jakob produced one clone with GFP-pbd inside but without PR. So I'm trying again to clone with two different PRs with different RBS strenghts.
Stored in: "Minipreps, verdaut"-box

                       

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