Team:EPF-Lausanne/Notebook/August2011

From 2011.igem.org

(Difference between revisions)
(Monday, 15 August 2011)
(Monday, 15 August 2011)
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All the LB bottles were contaminated therefore Alessandro made new ones. By now just one bottle at a time should be used by iGEMers in order to avoid multiple contamination. (take the one labeled "use me")
All the LB bottles were contaminated therefore Alessandro made new ones. By now just one bottle at a time should be used by iGEMers in order to avoid multiple contamination. (take the one labeled "use me")
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Alessandro ran the gel for the extension PCR on RFP and for gene-specific PCR on T4. In the picture there's the result: we miss a signal from T7lac-RFP and we have a weak signal from T7lac2-RFP therefore these two reactions have been repeated succesfully (see picture below).
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Alessandro ran the gel for the extension PCR on RFP and for gene-specific PCR on T4. In the picture there's the result: we miss a signal from T7lac-RFP and we have a weak signal from T7lac2-RFP therefore these two reactions have been repeated succesfully (see picture on the left).
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[[File:EPFL2011_extPCR2_150811.jpg|thumb|100px]]
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[[File:EPFL2011_extPCR2_150811.jpg|thumb|150px|left]]
Alessandro made PCRs to amplify pSB3K1 and pSB3C5 plasmids to use them as backbone for our device (T7-lysis/RFP) and they'll be ran on gel the day next. The extension PCR for T7-lysis failed because extension time was erroneously set as 1 minute instead of 2 minutes.
Alessandro made PCRs to amplify pSB3K1 and pSB3C5 plasmids to use them as backbone for our device (T7-lysis/RFP) and they'll be ran on gel the day next. The extension PCR for T7-lysis failed because extension time was erroneously set as 1 minute instead of 2 minutes.
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Revision as of 15:38, 15 August 2011