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Team:EPF-Lausanne/Todo
From 2011.igem.org
(Difference between revisions)
(→Preparing the parts) |
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| Line 12: | Line 12: | ||
Next steps: | Next steps: | ||
| - | * Make competent cells | + | * <s>Make competent cells</s> |
| - | + | ||
| - | + | ||
* Transform in E. Coli. | * Transform in E. Coli. | ||
* Glycerol Stocks | * Glycerol Stocks | ||
* Plasmid preps | * Plasmid preps | ||
| - | * | + | * Sequence the lysis cassette |
| + | |||
| + | == Assembly == | ||
| + | * Research plasmid backbones. Two different types are needed. | ||
| + | * Design Gibson primers to assemble the three different plasmids | ||
| + | * Determine which sequence on plasmids "two" and "three" should be used to create the "mega-plasmid" | ||
== Wiki == | == Wiki == | ||
* Start uploading protocols | * Start uploading protocols | ||
* Write-up team presentation | * Write-up team presentation | ||
| + | * Activate Javascript accordeon menu | ||
| + | * Fix alignement | ||
== Clean rooms == | == Clean rooms == | ||
* Order lab notebook | * Order lab notebook | ||
* Order storage box | * Order storage box | ||
Revision as of 16:12, 24 May 2011
{{{title}}}
Contents |
Todo
Microfluidic Chips
- Continue alignment training
Preparing the parts
We need to sequence-verify the lysis brick and LacI.
Status: Primers for sequencing the lysis brick have been ordered; reaction solutions for LacI sequencing have been prepared (ready to send out?).
Next steps:
-
Make competent cells - Transform in E. Coli.
- Glycerol Stocks
- Plasmid preps
- Sequence the lysis cassette
Assembly
- Research plasmid backbones. Two different types are needed.
- Design Gibson primers to assemble the three different plasmids
- Determine which sequence on plasmids "two" and "three" should be used to create the "mega-plasmid"
Wiki
- Start uploading protocols
- Write-up team presentation
- Activate Javascript accordeon menu
- Fix alignement
Clean rooms
- Order lab notebook
- Order storage box
