"
Team:Imperial College London/Project/Switch/Results
From 2011.igem.org
| Line 1: | Line 1: | ||
{{:Team:Imperial_College_London/Templates/Header}} | {{:Team:Imperial_College_London/Templates/Header}} | ||
| + | |||
| + | <html> | ||
| + | |||
| + | The first step was to extract the required biobricks from the registry, as we would need them many times each during the assembly. The part numbers and their locations in the 2011 distribution were looked up and then the DNA extracted by dissolving it in distilled water. We used RBS-RFP and the Cell Lysis Cassette from Berkeley 2008. | ||
| + | <p> | ||
| + | This DNA was used to transform competent 5-alpha cells. The cells were transformed according to the protocol on the main page and then transformants were selected for by growing on ampicillin plates. The colonies which grew were picked, grown for around 8 hours in LB broth at 37°C and then mini-prepped. The DNA obtained from the mini-prep was stored on ice overnight. | ||
| + | <p> | ||
| + | The next day, the DNA was digested using EcoRI and PstI, individually and together. The resulting digests were mixed with loading buffer and then run on an agarose gel at 90V for an hour. | ||
| + | <p> | ||
Revision as of 07:50, 15 August 2011
The first step was to extract the required biobricks from the registry, as we would need them many times each during the assembly. The part numbers and their locations in the 2011 distribution were looked up and then the DNA extracted by dissolving it in distilled water. We used RBS-RFP and the Cell Lysis Cassette from Berkeley 2008.
This DNA was used to transform competent 5-alpha cells. The cells were transformed according to the protocol on the main page and then transformants were selected for by growing on ampicillin plates. The colonies which grew were picked, grown for around 8 hours in LB broth at 37°C and then mini-prepped. The DNA obtained from the mini-prep was stored on ice overnight.
The next day, the DNA was digested using EcoRI and PstI, individually and together. The resulting digests were mixed with loading buffer and then run on an agarose gel at 90V for an hour.
