Revision as of 12:14, 12 August 2011

Team IGEM Paris 2011

Parameters

Description	Value	Unit	Reference
Concentration of X	NA	molecules per cell	Notation convention
Delay due to protein X production and maturation	NA	s	Notation convention
Maximal production rate of pVeg promoter (constitutive)	???	molecules.s^-1 or pops	Estimated
Maximal production rate of pLac promoter	0.02	molecules.s^-1 or pops	Estimated
Maximal production rate of pT7 promoter	0.02	molecules.s^-1 or pops	Estimated
Dissociation constant for IPTG to LacI	1200	molecules per cell	Aberdeen 2009 wiki
Dissociation constant for LacI to LacO (pLac)	700	molecules per cell	Aberdeen 2009 wiki
Dissociation constant for T7 RNA polymerase to pT7	3	molecules per cell	Estimated ADD EXPLANATION
Translation rate of proteins	1 or 0.9?	s^-1	Estimated ADD EXPLANATION
Dilution rate in exponential phase	2.88x10^-4	s^-1	Calculated with a 40 min generation time. See explanation
Degradation rate of mRNA	2.88x10^-3	s^-1	Uri Alon (To Be Confirmed)
Delay due tT7 RNA polymerase production and maturation	300	s	http://mol-biol4masters.masters.grkraj.org/html/Prokaryotic_DNA_Replication13-T7_Phage_DNA_Replication.htm
Delay due to mRNA production	30	s	http://bionumbers.hms.harvard.edu/bionumber.aspx?s=y&id=104902&ver=5&hlid=58815 2kb/(50b/s) --> approximation: all our contructs are around 2kb

@@ Line 70: / Line 70: @@
 <td><img src='https://static.igem.org/mediawiki/2011/a/a3/Gamma_protein.png' style='height:22px;'  /></td>
 <td>Translation rate of proteins</td>
-<td>1</td>
+<td>1 or 0.9?</td>
 <td>s<sup>-1</sup></td>
 <td>Estimated ADD EXPLANATION</td>