Team:EPF-Lausanne/Notebook/August2011

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(Wednesday, 10 August 2011)
(Wednesday, 10 August 2011)
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Henrike and Alessandro designed primers to put the T4 lysis cassette (BBa_K112808) under the control of the T7 promoter using as backbone: pSB3C5, pSB3K1, pSB3K5. This device will be used to test plasmid DNA recovery after lysis.
Henrike and Alessandro designed primers to put the T4 lysis cassette (BBa_K112808) under the control of the T7 promoter using as backbone: pSB3C5, pSB3K1, pSB3K5. This device will be used to test plasmid DNA recovery after lysis.
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Nadine designed primers to add Ptet-LacI in the pSB3K1-Pconst-TetR palsmid, also adding a terminator before LacI. We decided not to inculde the ssrA degardation tag in LacI, to be sure that the cells won't lyse.
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Since the pSB3K1 plasmid contains RFP, Nadine tried to cut it out by digesting it with XbaI and SpeI. Tomorrow we'll make a ligation.
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Revision as of 15:52, 10 August 2011