Team:EPF-Lausanne/Protocols/PDMS two layer device fabrication

From 2011.igem.org

(Difference between revisions)
(Method)
(Method)
Line 10: Line 10:
== Method ==
== Method ==
-
<p>• Place [[Team:EPF-Lausanne/Protocols/Master microfabrication for PDMS replica molding |molds]] into a TMCS vapor chamber</p>
+
# Place [[Team:EPF-Lausanne/Protocols/Master microfabrication for PDMS replica molding |molds]] into a TMCS vapor chamber
-
<p>• Control layer mixture: 20g Base + 4g Curing agent</p>
+
# Control layer mixture: 20g Base + 4g Curing agent
-
<p>• Mix for 1 minute, degas for 2 minutes (standard protocol)</p>
+
# Mix for 1 minute, degas for 2 minutes (standard protocol)
-
<p>• pour onto control layer mold and place mold in vacuum chamber</p>
+
#      Pour onto control layer mold and place mold in vacuum chamber
-
<p>• Flow layer mixture: 20g Base + 1g Curing agent</p>
+
#      Flow layer mixture: 20g Base + 1g Curing agent
-
<p>• Mix for 1 minute and degas for 2 minutes (standard protocol)</p>
+
# Mix for 1 minute and degas for 2 minutes (standard protocol)
-
<p>• Spin coat onto flow layer at 2200-2400rpm for 35secs (so far you can use my protocol on a spin coater)</p>
+
# Spin coat onto flow layer at 2200-2400rpm for 35secs  
-
<p>• Remove control layer mold from vacuum chamber, making sure no bubbles are left on the surface (remove with a toothpick if you see some )</p>
+
# Remove control layer mold from vacuum chamber, making sure no bubbles are left on the surface (remove with a toothpick if you see some )
-
<p>• Place the control and flow layer in a 80C convection oven and incubate for 30 minutes (timing is critical here!)</p>
+
# Place the control and flow layer in a 80C convection oven and incubate for 30 minutes (timing is critical here!)
-
<p>• Remove casts from oven, cut out control layer, punch holes, and align to flow layer</p>
+
# Remove casts from oven, cut out control layer, punch holes, and align to flow layer
-
<p>• Put aligned device back into 80C oven and incubate for at least 90 minutes (and here you can increase the backing time)</p>
+
# Put aligned device back into 80C oven and incubate for at least 90 minutes (and here you can increase the backing time)
-
<p>• Remove devices from oven and punch flow layer holes</p>
+
# Remove devices from oven and punch flow layer holes
{{:Team:EPF-Lausanne/Templates/Footer}}
{{:Team:EPF-Lausanne/Templates/Footer}}

Revision as of 07:36, 20 September 2011