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Copenhagen/4 August 2011
From 2011.igem.org
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==Thursday== | ==Thursday== | ||
| - | ''Lab Work'' | + | '''Lab Work''' |
* Run PCR on 1.4 (pink) to check the B1 primers, because they haven't worked yet. We used to much DNA, so we have to do one more time on monday. | * Run PCR on 1.4 (pink) to check the B1 primers, because they haven't worked yet. We used to much DNA, so we have to do one more time on monday. | ||
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| - | ''Other Work'' | + | '''Other Work''' |
* Studied on the requirements for the characterization of the BioBricks. | * Studied on the requirements for the characterization of the BioBricks. | ||
Latest revision as of 13:22, 4 August 2011
Thursday
Lab Work
- Run PCR on 1.4 (pink) to check the B1 primers, because they haven't worked yet. We used to much DNA, so we have to do one more time on monday.
- Double digest of 2.23.3 and 2.23.4 (purple)follwed by gelelectroforesis, byt it seems like, they digest havn't worked, so this step has to be done again.
- Transformation of A1,A2/Exp. vector into BL21 cells and A2,B1/pSB1C3 into XLblue cells.
Other Work
- Studied on the requirements for the characterization of the BioBricks.
- Meeting with Michael Lyngkjær about inducible E.coli promoters.
- Studied on USER cloning and primer design.
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