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Copenhagen/2 August 2011
From 2011.igem.org
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==Tuesday== | ==Tuesday== | ||
| - | + | We are very excited about the gel we are going to make today!! | |
'''Labwork''' | '''Labwork''' | ||
* Run gel on the PCR products from yesterday - to see if the new primers work better so we now will se an insert. The gel showed a positive result for A1 and A2 in the expr. vector. | * Run gel on the PCR products from yesterday - to see if the new primers work better so we now will se an insert. The gel showed a positive result for A1 and A2 in the expr. vector. | ||
| - | |||
* Make MiniPrep on the O/N cultures from yesterday which appear to have an insert - we have picked two of each of A1 and A2 which showed a posítive gel result. | * Make MiniPrep on the O/N cultures from yesterday which appear to have an insert - we have picked two of each of A1 and A2 which showed a posítive gel result. | ||
| - | |||
* Do digestion of PSB1C3 once again, since the digestion O/N was to be in 37 degrees. | * Do digestion of PSB1C3 once again, since the digestion O/N was to be in 37 degrees. | ||
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| + | '''Other work''' | ||
| + | |||
| + | * Renew projectdescription | ||
| + | |||
| + | * Find a fungi induced promoter | ||
Back to [[Team:Copenhagen/Notebook|Notebook]] | Back to [[Team:Copenhagen/Notebook|Notebook]] | ||
Revision as of 09:49, 2 August 2011
Tuesday
We are very excited about the gel we are going to make today!!
Labwork
- Run gel on the PCR products from yesterday - to see if the new primers work better so we now will se an insert. The gel showed a positive result for A1 and A2 in the expr. vector.
- Make MiniPrep on the O/N cultures from yesterday which appear to have an insert - we have picked two of each of A1 and A2 which showed a posítive gel result.
- Do digestion of PSB1C3 once again, since the digestion O/N was to be in 37 degrees.
Other work
- Renew projectdescription
- Find a fungi induced promoter
Back to Notebook
