Team:Sevilla/Week3

From 2011.igem.org

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<p>Monday 1 July</p>
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<p>Monday 25 July</p>
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<p>
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We spend the whole day planning our next step, given that the results call for a different approach. Designing primers, evaluating assembling methods... We leave some inoculums in order to start over with the minipreps and all that stuff tomorrow.
+
We spend the whole day planning our next step, given that the results call for a different approach. Designing primers, evaluating assembly methods... We leave some inoculums so we can start over with the mini-preps and all that stuff tomorrow.
</p>
</p>
<p>
<p>
What a tragedy! Our wonderful clock has fallen off the wall with a loud crash and it has smashed to pieces. RIP, dear pink clock.
What a tragedy! Our wonderful clock has fallen off the wall with a loud crash and it has smashed to pieces. RIP, dear pink clock.
</p>
</p>
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<img src="https://lh5.googleusercontent.com/-wJ3Ojic9TFc/Ti_tMfNw_mI/AAAAAAAAAc4/3w_BaQcz9Fo/s400/IMG_4779.JPG" style=padding-left:200px;"height="267" width="400" /></a>
<img src="https://lh5.googleusercontent.com/-wJ3Ojic9TFc/Ti_tMfNw_mI/AAAAAAAAAc4/3w_BaQcz9Fo/s400/IMG_4779.JPG" style=padding-left:200px;"height="267" width="400" /></a>
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Minipreps, digestions, etc. After using the NanoDrop to measure the DNA contained in our minipreprs we come to the conclusion that this machine is absolutely useless. Nothing like the classical electrophoresis to check whether you've isolated plasmids propperly!
+
Minipreps, digestions, etc. After using the NanoDrop to measure the DNA contained in our mini-preprs we come to the conclusion that this machine is absolutely useless. Nothing like the classical electrophoresis to check whether you've isolated plasmids properly!
</p>
</p>
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<p>
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XGal and IPTG have come in a box with 4 kg of dry ice. You know what that means?? A 15-MINUTE-PARTY IN THE LAB! (and a broken glass as a result of a bad experiment, but don't panic, nobody's been hurt). Erlenmeyers, graduates and glass bottles have also arrived today, with a big delay, but better late than never!
+
XGal and IPTG have come in a box with 4 kg of dry ice. You know what that means?? A 15-MINUTE-PARTY IN THE LAB! (and a broken glass as a result of an experiment gone wrong, but don't panic, nobody's been hurt). Erlenmeyers, graduates and glass bottles have also arrived today; very delayed, but better late than never!
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<p>Thursday</p>
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<p>Thursday 28 July</p>
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Revision as of 12:28, 29 July 2011


Monday 25 July


We spend the whole day planning our next step, given that the results call for a different approach. Designing primers, evaluating assembly methods... We leave some inoculums so we can start over with the mini-preps and all that stuff tomorrow.

What a tragedy! Our wonderful clock has fallen off the wall with a loud crash and it has smashed to pieces. RIP, dear pink clock.


Monday Protocols


In. Mid vel vel elementum, placerat tincidunt, vut lacus purus nunc massa a habitasse? Ac pid elementum aliquam, natoque! Porttitor facilisis amet lacus, ridiculus nascetur! Sociis vel aenean natoque penatibus aliquam in, augue mauris, dictumst, nunc mid platea a! Massa? Vut dis purus. Odio magna nec. Lorem ut turpis adipiscing scelerisque dis, scelerisque. Sed quis, porttitor et pulvinar in. Massa sit, porta urna nunc auctor non elementum! Augue dolor cum sociis, dignissim elementum. Integer. Penatibus. Phasellus et porta nec! Hac in nec porttitor ridiculus rhoncus. Hac natoque mid est nunc platea nisi sed habitasse! Amet nec placerat ultricies egestas. Vel cum diam, lorem integer et montes, nec placerat, turpis lacus nec placerat, rhoncus rhoncus, a porta augue ac! Et aliquet. Porttitor diam egestas, non vut etiam montes, aliquet? Pellentesque. Dolor nascetur amet. Adipiscing sed pulvinar cursus! Urna in, in turpis duis in sit placerat, vut non aliquam? Mattis habitasse augue mus. Pulvinar auctor natoque est lacus porta sagittis! A in massa lundium? In non, lundium nisi magna proin elementum sed porttitor sed amet risus a aliquet mid a? Proin turpis, ac magna, dictumst lorem tincidunt penatibus sociis rhoncus? Duis cursus sit diam amet nisi mattis egestas habitasse diam.


Tuesday 26 July


Minipreps, digestions, etc. After using the NanoDrop to measure the DNA contained in our mini-preprs we come to the conclusion that this machine is absolutely useless. Nothing like the classical electrophoresis to check whether you've isolated plasmids properly!


Tuesday Protocols


In. Mid vel vel elementum, placerat tincidunt, vut lacus purus nunc massa a habitasse? Ac pid elementum aliquam, natoque! Porttitor facilisis amet lacus, ridiculus nascetur! Sociis vel aenean natoque penatibus aliquam in, augue mauris, dictumst, nunc mid platea a! Massa? Vut dis purus. Odio magna nec. Lorem ut turpis adipiscing scelerisque dis, scelerisque. Sed quis, porttitor et pulvinar in. Massa sit, porta urna nunc auctor non elementum! Augue dolor cum sociis, dignissim elementum. Integer. Penatibus. Phasellus et porta nec! Hac in nec porttitor ridiculus rhoncus. Hac natoque mid est nunc platea nisi sed habitasse! Amet nec placerat ultricies egestas. Vel cum diam, lorem integer et montes, nec placerat, turpis lacus nec placerat, rhoncus rhoncus, a porta augue ac! Et aliquet. Porttitor diam egestas, non vut etiam montes, aliquet? Pellentesque. Dolor nascetur amet. Adipiscing sed pulvinar cursus! Urna in, in turpis duis in sit placerat, vut non aliquam? Mattis habitasse augue mus. Pulvinar auctor natoque est lacus porta sagittis! A in massa lundium? In non, lundium nisi magna proin elementum sed porttitor sed amet risus a aliquet mid a? Proin turpis, ac magna, dictumst lorem tincidunt penatibus sociis rhoncus? Duis cursus sit diam amet nisi mattis egestas habitasse diam.


Wednesday 27 July


XGal and IPTG have come in a box with 4 kg of dry ice. You know what that means?? A 15-MINUTE-PARTY IN THE LAB! (and a broken glass as a result of an experiment gone wrong, but don't panic, nobody's been hurt). Erlenmeyers, graduates and glass bottles have also arrived today; very delayed, but better late than never!


Wednesday Protocols


In. Mid vel vel elementum, placerat tincidunt, vut lacus purus nunc massa a habitasse? Ac pid elementum aliquam, natoque! Porttitor facilisis amet lacus, ridiculus nascetur! Sociis vel aenean natoque penatibus aliquam in, augue mauris, dictumst, nunc mid platea a! Massa? Vut dis purus. Odio magna nec. Lorem ut turpis adipiscing scelerisque dis, scelerisque. Sed quis, porttitor et pulvinar in. Massa sit, porta urna nunc auctor non elementum! Augue dolor cum sociis, dignissim elementum. Integer. Penatibus. Phasellus et porta nec! Hac in nec porttitor ridiculus rhoncus. Hac natoque mid est nunc platea nisi sed habitasse! Amet nec placerat ultricies egestas. Vel cum diam, lorem integer et montes, nec placerat, turpis lacus nec placerat, rhoncus rhoncus, a porta augue ac! Et aliquet. Porttitor diam egestas, non vut etiam montes, aliquet? Pellentesque. Dolor nascetur amet. Adipiscing sed pulvinar cursus! Urna in, in turpis duis in sit placerat, vut non aliquam? Mattis habitasse augue mus. Pulvinar auctor natoque est lacus porta sagittis! A in massa lundium? In non, lundium nisi magna proin elementum sed porttitor sed amet risus a aliquet mid a? Proin turpis, ac magna, dictumst lorem tincidunt penatibus sociis rhoncus? Duis cursus sit diam amet nisi mattis egestas habitasse diam.


Thursday 28 July


Vel cum diam, lorem integer et montes, nec placerat, turpis lacus nec placerat, rhoncus rhoncus, a porta augue ac! Et aliquet. Porttitor diam egestas, non vut etiam montes, aliquet? Pellentesque. Dolor nascetur amet. Adipiscing sed pulvinar cursus! Urna in, in turpis duis in sit placerat, vut non aliquam? Mattis habitasse augue mus. Pulvinar auctor natoque est lacus porta sagittis! A in massa lundium? In non, lundium nisi magna proin elementum sed porttitor sed amet risus a aliquet mid a? Proin turpis, ac magna, dictumst lorem tincidunt penatibus sociis rhoncus? Duis cursus sit diam amet nisi mattis egestas habitasse diam.


Thursday Protocols


In. Mid vel vel elementum, placerat tincidunt, vut lacus purus nunc massa a habitasse? Ac pid elementum aliquam, natoque! Porttitor facilisis amet lacus, ridiculus nascetur! Sociis vel aenean natoque penatibus aliquam in, augue mauris, dictumst, nunc mid platea a! Massa? Vut dis purus. Odio magna nec. Lorem ut turpis adipiscing scelerisque dis, scelerisque. Sed quis, porttitor et pulvinar in. Massa sit, porta urna nunc auctor non elementum! Augue dolor cum sociis, dignissim elementum. Integer. Penatibus. Phasellus et porta nec! Hac in nec porttitor ridiculus rhoncus. Hac natoque mid est nunc platea nisi sed habitasse! Amet nec placerat ultricies egestas. Vel cum diam, lorem integer et montes, nec placerat, turpis lacus nec placerat, rhoncus rhoncus, a porta augue ac! Et aliquet. Porttitor diam egestas, non vut etiam montes, aliquet? Pellentesque. Dolor nascetur amet. Adipiscing sed pulvinar cursus! Urna in, in turpis duis in sit placerat, vut non aliquam? Mattis habitasse augue mus. Pulvinar auctor natoque est lacus porta sagittis! A in massa lundium? In non, lundium nisi magna proin elementum sed porttitor sed amet risus a aliquet mid a? Proin turpis, ac magna, dictumst lorem tincidunt penatibus sociis rhoncus? Duis cursus sit diam amet nisi mattis egestas habitasse diam.


Friday


wwwwwwwwwwwwwwwwRhoncus nisi adipiscing, rhoncus. Porttitor urna rhoncus? A placerat scelerisque mattis lectus tristique magna pellentesque ac ac, et? Adipiscing penatibus porta. Turpis ultrices lorem vel vel massa elementum! Lorem vel lectus et integer porttitor magna aenean, quis magnis turpis aliquam? Sed enim? Placerat velit sit turpis penatibus enim. Enim cursus hac massa eu a cum etiam? Amet pid porta non tincidunt cum pellentesque eu a pid nisi ultrices? Lectus augue dictumst purus, velit scelerisque vut. A porttitor turpis, natoque aliquam elementum. Tristique, mauris turpis? Arcu tristique sed massa est nunc purus pellentesque, risus nec rhoncus, scelerisque ac urna porttitor, sit.


Friday Protocols


In. Mid vel vel elementum, placerat tincidunt, vut lacus purus nunc massa a habitasse? Ac pid elementum aliquam, natoque! Porttitor facilisis amet lacus, ridiculus nascetur! Sociis vel aenean natoque penatibus aliquam in, augue mauris, dictumst, nunc mid platea a! Massa? Vut dis purus. Odio magna nec. Lorem ut turpis adipiscing scelerisque dis, scelerisque. Sed quis, porttitor et pulvinar in. Massa sit, porta urna nunc auctor non elementum! Augue dolor cum sociis, dignissim elementum. Integer. Penatibus. Phasellus et porta nec! Hac in nec porttitor ridiculus rhoncus. Hac natoque mid est nunc platea nisi sed habitasse! Amet nec placerat ultricies egestas. Vel cum diam, lorem integer et montes, nec placerat, turpis lacus nec placerat, rhoncus rhoncus, a porta augue ac! Et aliquet. Porttitor diam egestas, non vut etiam montes, aliquet? Pellentesque. Dolor nascetur amet. Adipiscing sed pulvinar cursus! Urna in, in turpis duis in sit placerat, vut non aliquam? Mattis habitasse augue mus. Pulvinar auctor natoque est lacus porta sagittis! A in massa lundium? In non, lundium nisi magna proin elementum sed porttitor sed amet risus a aliquet mid a? Proin turpis, ac magna, dictumst lorem tincidunt penatibus sociis rhoncus? Duis cursus sit diam amet nisi mattis egestas habitasse diam.


Weekend


wwwwwwwwwwwwwwwwRhoncus nisi adipiscing, rhoncus. Porttitor urna rhoncus? A placerat scelerisque mattis lectus tristique magna pellentesque ac ac, et? Adipiscing penatibus porta. Turpis ultrices lorem vel vel massa elementum! Lorem vel lectus et integer porttitor magna aenean, quis magnis turpis aliquam? Sed enim? Placerat velit sit turpis penatibus enim. Enim cursus hac massa eu a cum etiam? Amet pid porta non tincidunt cum pellentesque eu a pid nisi ultrices? Lectus augue dictumst purus, velit scelerisque vut. A porttitor turpis, natoque aliquam elementum. Tristique, mauris turpis? Arcu tristique sed massa est nunc purus pellentesque, risus nec rhoncus, scelerisque ac urna porttitor, sit.


Weekend Protocols


In. Mid vel vel elementum, placerat tincidunt, vut lacus purus nunc massa a habitasse? Ac pid elementum aliquam, natoque! Porttitor facilisis amet lacus, ridiculus nascetur! Sociis vel aenean natoque penatibus aliquam in, augue mauris, dictumst, nunc mid platea a! Massa? Vut dis purus. Odio magna nec. Lorem ut turpis adipiscing scelerisque dis, scelerisque. Sed quis, porttitor et pulvinar in. Massa sit, porta urna nunc auctor non elementum! Augue dolor cum sociis, dignissim elementum. Integer. Penatibus. Phasellus et porta nec! Hac in nec porttitor ridiculus rhoncus. Hac natoque mid est nunc platea nisi sed habitasse! Amet nec placerat ultricies egestas. Vel cum diam, lorem integer et montes, nec placerat, turpis lacus nec placerat, rhoncus rhoncus, a porta augue ac! Et aliquet. Porttitor diam egestas, non vut etiam montes, aliquet? Pellentesque. Dolor nascetur amet. Adipiscing sed pulvinar cursus! Urna in, in turpis duis in sit placerat, vut non aliquam? Mattis habitasse augue mus. Pulvinar auctor natoque est lacus porta sagittis! A in massa lundium? In non, lundium nisi magna proin elementum sed porttitor sed amet risus a aliquet mid a? Proin turpis, ac magna, dictumst lorem tincidunt penatibus sociis rhoncus? Duis cursus sit diam amet nisi mattis egestas habitasse diam.

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