Team:Freiburg/Notebook/18 July

From 2011.igem.org

(Difference between revisions)
(→NAME OF YOUR EXPERIMENT)
Line 102: Line 102:
What program do you use?
What program do you use?
-
10x (95C-41/52C-72C) + 25x ((95C-60C-72C)
+
10x (95C-41C/47C/52C-72C) + 25x ((95C-60C-72C)
Line 109: Line 109:
Reactions:
Reactions:
 +
 +
P28+P18+M14.1
 +
 +
P28+P19+M14.1
 +
 +
P28+P20+M14.1
 +
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P28+P2+M14.1
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P28+P3+M14.1
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P1+P18+M14.1
P1+P18+M14.1
Line 121: Line 132:
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P28+P18+M14.1
 
-
P28+P19+M14.1
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P1+P3+M14.2
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P28+P20+M14.1
 
-
P28+P2+M14.1
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[[File:Freiburg11_Phusion_ladder.JPG]]
-
P28+P3+M14.1
 
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[[File:Freiburg11_7_19_2011_7_31_40_PM.Jpg]]
-
P1+P3+M14.2
+
'''Upper panel:'''All lanes at 41C
 +
 
 +
{| style="border-spacing:0;"
 +
| style="border-top:0.0007in solid #000000;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| '''Lane 1'''
 +
| style="border-top:0.0007in solid #000000;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| '''Lane 2'''
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| style="border-top:0.0007in solid #000000;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| '''Lane 3'''
 +
| style="border-top:0.0007in solid #000000;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| '''Lane 4'''
 +
| style="border-top:0.0007in solid #000000;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| '''Lane 5'''
 +
| style="border-top:0.0007in solid #000000;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| '''Lane 6'''
 +
| style="border-top:0.0007in solid #000000;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| '''Lane 7'''
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| style="border-top:0.0007in solid #000000;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| '''Lane 8'''
 +
| style="border-top:0.0007in solid #000000;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| '''Lane 9'''
 +
| style="border:0.0007in solid #000000;padding:0.0382in;"| '''Lane 10'''
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 +
|-
 +
| style="border-top:none;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| Quick Load Marker
 +
| style="border-top:none;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| P28+P18+M14.1
 +
| style="border-top:none;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| P28+P19+M14.1
 +
| style="border-top:none;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| P28+P20+M14.1
 +
| style="border-top:none;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| P28+P2+M14.1
 +
| style="border-top:none;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"|
 +
| style="border-top:none;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| P1+P19+M14.1
 +
| style="border-top:none;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"|
 +
| style="border-top:none;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| P1+P20+M14.1
 +
| style="border-top:none;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:0.0007in solid #000000;padding:0.0382in;"| P1+P2+M14.1
 +
 
 +
 
 +
 
 +
 
 +
|}
 +
'''Lower Panel'''
 +
 
 +
 
 +
{| style="border-spacing:0;"
 +
| style="border-top:0.0007in solid #000000;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| '''Lane 1'''
 +
| style="border-top:0.0007in solid #000000;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| '''Lane 2'''
 +
| style="border-top:0.0007in solid #000000;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| '''Lane 3'''
 +
| style="border-top:0.0007in solid #000000;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| '''Lane 4'''
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| style="border-top:0.0007in solid #000000;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| '''Lane 5'''
 +
| style="border-top:0.0007in solid #000000;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| '''Lane 6'''
 +
| style="border-top:0.0007in solid #000000;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| '''Lane 7'''
 +
| style="border-top:0.0007in solid #000000;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| '''Lane 8'''
 +
| style="border-top:0.0007in solid #000000;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| '''Lane 9'''
 +
| style="border:0.0007in solid #000000;padding:0.0382in;"| '''Lane 10'''
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 +
|-
 +
| style="border-top:none;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| Quick Load Marker
 +
| style="border-top:none;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| P1+P3+M14.1
 +
 
 +
41C
 +
| style="border-top:none;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| P1+P3+M14.1
 +
 
 +
47C
 +
| style="border-top:none;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| P1+P3+M14.2
 +
 
 +
52C
 +
| style="border-top:none;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| P1+P3+M14.1
 +
 
 +
52C
 +
| style="border-top:none;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| P28+P3+M14.1
 +
 
 +
41C
 +
| style="border-top:none;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| P1+P18+M14.1
 +
 
 +
41C
 +
| style="border-top:none;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| P28+P3+M14.1
 +
 
 +
47C
 +
| style="border-top:none;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| P28+P3+M14.1
 +
 
 +
52C
 +
| style="border-top:none;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:0.0007in solid #000000;padding:0.0382in;"|
 +
 
 +
|}
 +
'''Upper Panel: '''all at 47C
 +
 
 +
 
 +
{| style="border-spacing:0;"
 +
| style="border-top:0.0007in solid #000000;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| '''Lane 1'''
 +
| style="border-top:0.0007in solid #000000;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| '''Lane 2'''
 +
| style="border-top:0.0007in solid #000000;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| '''Lane 3'''
 +
| style="border-top:0.0007in solid #000000;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| '''Lane 4'''
 +
| style="border-top:0.0007in solid #000000;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| '''Lane 5'''
 +
| style="border-top:0.0007in solid #000000;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| '''Lane 6'''
 +
| style="border-top:0.0007in solid #000000;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| '''Lane 7'''
 +
| style="border-top:0.0007in solid #000000;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| '''Lane 8'''
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| style="border-top:0.0007in solid #000000;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| '''Lane 9'''
 +
| style="border:0.0007in solid #000000;padding:0.0382in;"| '''Lane 10'''
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 +
|-
 +
| style="border-top:none;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| Quick Load Marker
 +
| style="border-top:none;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| P28+P18+M14.1
 +
| style="border-top:none;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| P28+P19+M14.1
 +
| style="border-top:none;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| P28+P20+M14.1
 +
| style="border-top:none;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| P28+P2+M14.1
 +
| style="border-top:none;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"|
 +
| style="border-top:none;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| P1+P18+M14.1
 +
| style="border-top:none;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| P1+P19+M14.1
 +
| style="border-top:none;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| P1+P20+M14.1
 +
| style="border-top:none;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:0.0007in solid #000000;padding:0.0382in;"| P1+P2+M14.1
 +
 
 +
|}
 +
'''Lower Panel:''' all at 52C
 +
 
 +
 
 +
{| style="border-spacing:0;"
 +
| style="border-top:0.0007in solid #000000;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| '''Lane 1'''
 +
| style="border-top:0.0007in solid #000000;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| '''Lane 2'''
 +
| style="border-top:0.0007in solid #000000;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| '''Lane 3'''
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| style="border-top:0.0007in solid #000000;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| '''Lane 4'''
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| style="border-top:0.0007in solid #000000;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| '''Lane 5'''
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| style="border-top:0.0007in solid #000000;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| '''Lane 6'''
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| style="border-top:0.0007in solid #000000;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| '''Lane 7'''
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| style="border-top:0.0007in solid #000000;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| '''Lane 8'''
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| style="border-top:0.0007in solid #000000;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| '''Lane 9'''
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| style="border:0.0007in solid #000000;padding:0.0382in;"| '''Lane 10'''
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 +
|-
 +
| style="border-top:none;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| Quick Load Marker
 +
| style="border-top:none;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| P28+P18+M14.1
 +
| style="border-top:none;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| P28+P19+M14.1
 +
| style="border-top:none;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| P28+P20+M14.1
 +
| style="border-top:none;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| P28+P2+M14.1
 +
| style="border-top:none;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"|
 +
| style="border-top:none;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| P1+P18+M14.1
 +
| style="border-top:none;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| P1+P19+M14.1
 +
| style="border-top:none;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| P1+P20+M14.1
 +
| style="border-top:none;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:0.0007in solid #000000;padding:0.0382in;"| P1+P2+M14.1
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|}
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[[File:Freiburg11_7_19_2011_7_38_41_PM.Jpg]]

Revision as of 17:07, 19 July 2011

Contents

green light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME


blue light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME



red light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME



Lysis cassette

NAME OF YOUR EXPERIMENT

Investigators:NAME


Precipitator

NAME OF YOUR EXPERIMENT

PCR


Name:

Ruediger

Date:

18.07.2011

Continue from Experiment (Date)PCR 1507

(Name) Ruediger

Project Name:

GFP Pbd

PCR-Mixture for one Reaction:

For a 50 µl reaction use


32,5µl H20
10µl 5x Phusion Buffer
2.5µl Primer fw
2.5µl Primer dw
1µl dNTPs
1µl DNA-Template
0.5 µl Phusion (add in the end)

What program do you use?

10x (95C-41C/47C/52C-72C) + 25x ((95C-60C-72C)


How did you label the PCR-Product, where is it stored and what do you do next?


Reactions:

P28+P18+M14.1

P28+P19+M14.1

P28+P20+M14.1

P28+P2+M14.1

P28+P3+M14.1


P1+P18+M14.1

P1+P19+M14.1

P1+P20+M14.1

P1+P2+M14.1

P1+P3+M14.1


P1+P3+M14.2


Freiburg11 Phusion ladder.JPG


Freiburg11 7 19 2011 7 31 40 PM.Jpg

Upper panel:All lanes at 41C

Lane 1 Lane 2 Lane 3 Lane 4 Lane 5 Lane 6 Lane 7 Lane 8 Lane 9 Lane 10
Quick Load Marker P28+P18+M14.1 P28+P19+M14.1 P28+P20+M14.1 P28+P2+M14.1 P1+P19+M14.1 P1+P20+M14.1 P1+P2+M14.1



Lower Panel


Lane 1 Lane 2 Lane 3 Lane 4 Lane 5 Lane 6 Lane 7 Lane 8 Lane 9 Lane 10
Quick Load Marker P1+P3+M14.1

41C

P1+P3+M14.1

47C

P1+P3+M14.2

52C

P1+P3+M14.1

52C

P28+P3+M14.1

41C

P1+P18+M14.1

41C

P28+P3+M14.1

47C

P28+P3+M14.1

52C

Upper Panel: all at 47C


Lane 1 Lane 2 Lane 3 Lane 4 Lane 5 Lane 6 Lane 7 Lane 8 Lane 9 Lane 10
Quick Load Marker P28+P18+M14.1 P28+P19+M14.1 P28+P20+M14.1 P28+P2+M14.1 P1+P18+M14.1 P1+P19+M14.1 P1+P20+M14.1 P1+P2+M14.1

Lower Panel: all at 52C


Lane 1 Lane 2 Lane 3 Lane 4 Lane 5 Lane 6 Lane 7 Lane 8 Lane 9 Lane 10
Quick Load Marker P28+P18+M14.1 P28+P19+M14.1 P28+P20+M14.1 P28+P2+M14.1 P1+P18+M14.1 P1+P19+M14.1 P1+P20+M14.1 P1+P2+M14.1





File:Freiburg11 7 19 2011 7 38 41 PM.Jpg

Retrieved from "http://2011.igem.org/Team:Freiburg/Notebook/18_July"