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Team:Cornell/Week 6
From 2011.igem.org
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*RFP PCR done overnight | *RFP PCR done overnight | ||
*Sequencing revealed no GFP. Retry the 3-piece ligation of GFP + Annealed Primer Pair A (half of biotin tag) + Annealed Primer Pair B (other half of biotin tag & iGEM suffix). | *Sequencing revealed no GFP. Retry the 3-piece ligation of GFP + Annealed Primer Pair A (half of biotin tag) + Annealed Primer Pair B (other half of biotin tag & iGEM suffix). | ||
| + | *PCR to amplify vector backbone containing RFP. | ||
==Monday== | ==Monday== | ||
Revision as of 03:45, 18 July 2011
Week 1 | Week 2 - 5 | Week 6 | Week 7 | Week 8 | Week 9 | Week 10 | Week 11 | Week 12 | Week 13 | Week 14 | Week 15 | Week 16 | Week 17 | Week 18 | Week 19 | Week 20 | Week 21 |
June 10th - June 16th
Sunday
- Worked on Website. Preliminary design of banner, safety content, and temporary lab notebook spreadsheet.
- Ligation product was transformed in electrocompetent cells, culture plated on Amp
- Vio-Operon plasmid was transformed and plated on Kanamyacin
- RFP PCR done overnight
- Sequencing revealed no GFP. Retry the 3-piece ligation of GFP + Annealed Primer Pair A (half of biotin tag) + Annealed Primer Pair B (other half of biotin tag & iGEM suffix).
- PCR to amplify vector backbone containing RFP.
