Team:Freiburg/Notebook/14 July
From 2011.igem.org
(Difference between revisions)
(→PCR) |
(→3A-assembly: Digest) |
||
Line 34: | Line 34: | ||
*Enzyme 2: PstI | *Enzyme 2: PstI | ||
*Enzyme 3: DpnI | *Enzyme 3: DpnI | ||
+ | <br/> | ||
===3A-assembly: Ligation and transformation=== | ===3A-assembly: Ligation and transformation=== |
Revision as of 13:52, 15 July 2011
Contents |
green light receptor
NAME OF YOUR EXPERIMENT
Investigators:NAME
blue light receptor
NAME OF YOUR EXPERIMENT
Investigators:NAME
red light receptor
3A-assembly: Digest
Investigators: Jakob
3A-assembly of S22 (ho1)+S21a (terminator) and S23 (pcyA)+S21a (terminator)
S21a:
- Enzyme 1: XbaI
- Enzyme 2: PstI
S22, S23
- Enzyme 1: EcoRI
- Enzyme 2: SpeI
Vector:
- Enzyme 1: EcoRI
- Enzyme 2: PstI
- Enzyme 3: DpnI
3A-assembly: Ligation and transformation
Investigators: Julia
Ligation of S22 (ho1)+S21a (terminator) and S23 (pcyA)+S21a (terminator) in vector pSB1T3.
L23: Ligation of S23+S21a in pSB1T3 L22: Ligation of S22+S21a in pSB1T3
Plasmids were transformed in cells and plated out.
PCR
Investigators: Julia
PCR of the red light receptor, cph8.
Primer:
- P 10
- P 11
DNA template:
- BBa_I150010
Lysis cassette
NAME OF YOUR EXPERIMENT
Investigators:NAME
Precipitator
Test-digest
Investigators: Rüdiger
Testdigest of the minipreps, Sophie did (june, 13).
- Enzyme 1: PstI
- ENzyme 2: EcoRI
Image of different samples from miniprep.