Team:EPF-Lausanne/Protocols/Primers preparation

From 2011.igem.org

(Difference between revisions)
m
Line 4: Line 4:
When receiving new primers, we need to add water in them on order to achieve 50 microM concentration. One rapid method is to:
When receiving new primers, we need to add water in them on order to achieve 50 microM concentration. One rapid method is to:
* take the value "nmol yield" on the synthesis report and multiply it 20x to have the needed microliters of water
* take the value "nmol yield" on the synthesis report and multiply it 20x to have the needed microliters of water
-
* spin down the primers tube to be sure that all DNA is at the bottom
+
* '''spin down the primers tube''' to be sure that all DNA is at the bottom
* add the calculated amount of water in each tube, then mix them
* add the calculated amount of water in each tube, then mix them
{{:Team:EPF-Lausanne/Templates/Footer}}
{{:Team:EPF-Lausanne/Templates/Footer}}

Revision as of 14:31, 25 July 2011