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Team:Freiburg/Notebook/8 June
From 2011.igem.org
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==Amplification of different parts== | ==Amplification of different parts== | ||
Revision as of 07:22, 15 July 2011
Precipitator
Protein modelling with pymol
Investigators: Rüdiger
Amplification of different parts
Investigators: Julia and Jacob
antibiotics plates:
1oo mg/ml ampicillin (amp) (100 microliter per plate)
1oo mikroliter H2O + 2 microliter canamycin (cm)
1oo mikroliter H2O + 40 microliter tetracyclin(tet)
1oo mikroliter H2O + 10 microliter kanamycin (kn)
| part | location(p=plate) | resistance | info |
| BBa_K098995 | P3, 1E | amp | heat sensitive cI QPI with high promoter |
| BBa_K112022 | P3, 24E | amp | Lambda phage lysis device - no promoter, This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. |
| pSB1K3 | P1, 5A | kan | high copy BioBrick assemby plasmid |
| pSB1A3 | P1, 1G | amp | high copy BioBrick assemby plasmid |
| pSB1C3 | P1,3A | cm | high copy BioBrick assemby plasmid |
| pSB1T3 | P1, 7A | tet | high copy BioBrick assemby plasmid |
| J23104 | P1, 18K | promotor | |
| J23110 | P1, 20 C | promotor | |
| J23116 | P1, 20M | promotor | |
| B0034 | P1, 2M | RBS (strong) | |
| B0032 | P1, 2 I | RBS.3 (medium) | |
| B0031 | P1, 2G | RBS.2 (weak) |
Transformation of parts in competent cells and plating them out on plates with specific antiobiotic resistance.
