Thursday, July 14

(Difference between revisions)

Latest revision as of 18:41, 19 July 2011

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I loaded the gel as explained above. Gel electrophoresis G1 (10 lanes of; ladder, control A&B, Rxn 1 A&B, 4 A&B &5 A&B)

Start : 1.55 pm

Stop : 2.55 pm

Voltage : 140 v, 122 Amps

Freshened the ethidium bromide by putting the 100 micro liter (.1 gm/10ml of di water). Ethidium bromide was kept foiled and kept in dark freezer.

Rocking started at 3.00pm took off at 4.07pm and photographed it.

Gel electrophoresis G2 (4 lanes of; ladder, Rxn 2 A&B, and 3 A&B)

_____ _____ _____ _____ _____ Ladder Rxn 2B 3A 3B 6 micro ltr 2A

Started : 3.00pm Stopped : 4.15 pm Rocker start: 4.25 pm Took a picture of the gel.

For digestion of the PCR product Digestion did for 4A, 3A & 3B

40 micro ltr of the PCR product and 1 micro ltr of Dpn1 and vortex it. Kept it in hot bath at temp 37 oC for 60 mins. Started at : 9 pm

Hours

@@ Line 1: / Line 1: @@
-{{LabReportBmore}}
+[[File:gel1.jpg|200px|thumb|right|alt text]]
+[[File:gel2.jpg|300px|thumb|right|alt text]]
 I loaded the gel as explained above.
 Gel electrophoresis G1  (10 lanes of; ladder, control A&B, Rxn 1 A&B, 4 A&B &5 A&B)
-Start : 1.55 pm
-Stop : 2.55 pm
+: Start : 1.55 pm
+: Stop : 2.55 pm
 Voltage : 140 v, 122 Amps
-[[File:gel1.jpg|200px|thumb|left|alt text]]
 Freshened the ethidium bromide by putting the 100 micro liter (.1 gm/10ml of di water). Ethidium bromide was kept foiled and kept in dark freezer.
@@ Line 17: / Line 20: @@
 micro ltr		2A
-[[File:gel2.jpg|300px|thumb|left|alt text]]
 Started : 3.00pm
@@ Line 31: / Line 34: @@
 Kept it in hot bath at temp 37 oC  for 60 mins.
 Started at : 9 pm
+{{LabReportBmore}}