Thursday, July 14

From 2011.igem.org

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I loaded the gel as explained above. 
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Gel electrophoresis G1  (10 lanes of; ladder, control A&B, Rxn 1 A&B, 4 A&B &5 A&B)
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Start : 1.55 pm
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Stop : 2.55 pm
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Voltage : 140 v, 122 Amps
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[[File:gel1.jpg]]
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Freshened the ethidium bromide by putting the 100 micro liter (.1 gm/10ml of di water). Ethidium bromide was kept foiled and kept in dark freezer.
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Rocking started at 3.00pm took off at 4.07pm and photographed it.
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Gel electrophoresis G2  (4 lanes of; ladder, Rxn 2 A&B, and 3 A&B)
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_____ _____ _____ _____ _____
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Ladder Rxn 2B 3A 3B
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6 micro ltr 2A
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[[File:gel2.jpg]]
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Started : 3.00pm
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Stopped : 4.15 pm
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Rocker start: 4.25 pm
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Took a picture of the gel.
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For digestion of the PCR product
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Digestion did for
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4A, 3A & 3B
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40 micro ltr of the PCR product and 1 micro ltr of Dpn1 and vortex it.
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Kept it in hot bath at temp 37 oC  for 60 mins.
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Started at : 9 pm

Revision as of 01:31, 15 July 2011

Team:Baltimore/Notebook

Hours


I loaded the gel as explained above. Gel electrophoresis G1 (10 lanes of; ladder, control A&B, Rxn 1 A&B, 4 A&B &5 A&B) Start : 1.55 pm Stop : 2.55 pm Voltage : 140 v, 122 Amps Gel1.jpg

Freshened the ethidium bromide by putting the 100 micro liter (.1 gm/10ml of di water). Ethidium bromide was kept foiled and kept in dark freezer.

Rocking started at 3.00pm took off at 4.07pm and photographed it.

Gel electrophoresis G2 (4 lanes of; ladder, Rxn 2 A&B, and 3 A&B)

_____ _____ _____ _____ _____ Ladder Rxn 2B 3A 3B 6 micro ltr 2A

Gel2.jpg

Started : 3.00pm Stopped : 4.15 pm Rocker start: 4.25 pm Took a picture of the gel.

For digestion of the PCR product Digestion did for 4A, 3A & 3B

40 micro ltr of the PCR product and 1 micro ltr of Dpn1 and vortex it. Kept it in hot bath at temp 37 oC for 60 mins. Started at : 9 pm