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Team:Freiburg/Notebook/18 July
From 2011.igem.org
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===NAME OF YOUR EXPERIMENT=== | ===NAME OF YOUR EXPERIMENT=== | ||
| - | ''' | + | '''PCR''' |
| + | |||
| + | |||
| + | {| style="border-spacing:0;" | ||
| + | | style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| Name: | ||
| + | |||
| + | Ruediger | ||
| + | | style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| Date: | ||
| + | |||
| + | 18.07.2011 | ||
| + | |||
| + | |- | ||
| + | | colspan="2" style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| Continue from Experiment (Date)PCR 1507 | ||
| + | |||
| + | (Name) Ruediger | ||
| + | |||
| + | |- | ||
| + | | colspan="2" style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| Project Name: | ||
| + | |||
| + | GFP Pbd | ||
| + | |||
| + | |} | ||
| + | PCR-Mixture for one Reaction: | ||
| + | |||
| + | For a 50 µl reaction use | ||
| + | |||
| + | |||
| + | {| style="border-spacing:0;" | ||
| + | | style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| 32,5µl | ||
| + | | style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| H<sub>2</sub>0 | ||
| + | | style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| | ||
| + | |||
| + | |- | ||
| + | | style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| 10µl | ||
| + | | style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| 5x Phusion Buffer | ||
| + | | style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| | ||
| + | |||
| + | |- | ||
| + | | style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| 2.5µl | ||
| + | | style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| Primer fw | ||
| + | | style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| | ||
| + | |||
| + | |- | ||
| + | | style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| 2.5µl | ||
| + | | style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| Primer dw | ||
| + | | style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| | ||
| + | |||
| + | |- | ||
| + | | style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| 1µl | ||
| + | | style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| dNTPs | ||
| + | | style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| | ||
| + | |||
| + | |- | ||
| + | | style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| 1µl | ||
| + | | style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| DNA-Template | ||
| + | | style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| | ||
| + | |||
| + | |- | ||
| + | | style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| 0.5 µl | ||
| + | | style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| Phusion (add in the end) | ||
| + | | style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| | ||
| + | |||
| + | |} | ||
| + | What program do you use? | ||
| + | |||
| + | 10x (95C-41/52C-72C) + 25x ((95C-60C-72C) | ||
| + | |||
| + | |||
| + | How did you label the PCR-Product, where is it stored and what do you do next? | ||
| + | |||
| + | |||
| + | Reactions: | ||
| + | |||
| + | P1+P18+M14.1 | ||
| + | |||
| + | P1+P19+M14.1 | ||
| + | |||
| + | P1+P20+M14.1 | ||
| + | |||
| + | P1+P2+M14.1 | ||
| + | |||
| + | P1+P3+M14.1 | ||
| + | |||
| + | |||
| + | P28+P18+M14.1 | ||
| + | |||
| + | P28+P19+M14.1 | ||
| + | |||
| + | P28+P20+M14.1 | ||
| + | |||
| + | P28+P2+M14.1 | ||
| + | |||
| + | P28+P3+M14.1 | ||
| + | |||
| + | |||
| + | P1+P3+M14.2 | ||
Revision as of 14:24, 18 July 2011
Contents |
green light receptor
NAME OF YOUR EXPERIMENT
Investigators:NAME
blue light receptor
NAME OF YOUR EXPERIMENT
Investigators:NAME
red light receptor
NAME OF YOUR EXPERIMENT
Investigators:NAME
Lysis cassette
NAME OF YOUR EXPERIMENT
Investigators:NAME
Precipitator
NAME OF YOUR EXPERIMENT
PCR
| Name:
Ruediger | Date:
18.07.2011 |
| Continue from Experiment (Date)PCR 1507
(Name) Ruediger | |
| Project Name:
GFP Pbd | |
PCR-Mixture for one Reaction:
For a 50 µl reaction use
| 32,5µl | H20 | |
| 10µl | 5x Phusion Buffer | |
| 2.5µl | Primer fw | |
| 2.5µl | Primer dw | |
| 1µl | dNTPs | |
| 1µl | DNA-Template | |
| 0.5 µl | Phusion (add in the end) |
What program do you use?
10x (95C-41/52C-72C) + 25x ((95C-60C-72C)
How did you label the PCR-Product, where is it stored and what do you do next?
Reactions:
P1+P18+M14.1
P1+P19+M14.1
P1+P20+M14.1
P1+P2+M14.1
P1+P3+M14.1
P28+P18+M14.1
P28+P19+M14.1
P28+P20+M14.1
P28+P2+M14.1
P28+P3+M14.1
P1+P3+M14.2
