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Team:Freiburg/Notebook/21 June
From 2011.igem.org
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==Sequencing== | ==Sequencing== | ||
Revision as of 12:14, 14 July 2011
Sequencing
Primer concentration has to be 2 nM.
Dilution of stock: 2 μl in 100 μl H2O.
max 100 ng/μl of DNA.
| pos. clone | ng/μl | μl |
| PR1e | 46.8 | 2.1 |
| PR2d | 67.9 | 1.4 |
| PR3e | 45.8 | 2.2 |
| PR4b | 61.5 | 1.6 |
| PR5a | 74.5 | 1.3 |
| PR6c | 94.7 | 1.06 |
| PR7b | 69.1 | 1.4 |
| PR8b | 81.9 | 1.2 |
| PR9a | 64.4 | 1.5 |
| lys 1 | 16 | 10 |
| lys 2 | 21.7 | 10 |
| lys 3 | 2.5 | 10 |
| lys 4 | 16.4 | 10 |
| lys 5 | 10.2 | 10 |
