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Team:Northwestern/Notebook/Protocols/Overnight
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| - | *Take 5mL LB and put in a round bottom tube | + | *Take 5mL LB and put in a round bottom 5mL tube. |
| - | *If your antibiotic is 1000x (which it should be) add 5uL antibiotic to your tube | + | *If your antibiotic is 1000x (which it should be) add 5uL antibiotic to your tube. Make sure you add the right one! |
| - | + | *Take a tip on a p200 and lightly touch a colony. | |
| - | *Take a tip on a p200 and lightly touch a colony | + | *Eject the tip into the culture and put the cap on. |
| - | *Eject the tip into the culture and put the cap on | + | *For a miniprep, grow overnight for 12-16 hours NOT LONGER. |
| - | *For a miniprep, grow | + | |
| - | + | ||
'''REMEMBER TO USE PROPER STERILE TECHNIQUE''' | '''REMEMBER TO USE PROPER STERILE TECHNIQUE''' | ||
| + | |||
| + | Adapted from [http://openwetware.org/wiki/Bacterial_cell_culture OpenWetWare] | ||
Revision as of 20:51, 11 July 2011
PROJECT
RESULTS
CONSIDERATIONS
ABOUT US
NOTEBOOK
ATTRIBUTIONS
Overnight
- Take 5mL LB and put in a round bottom 5mL tube.
- If your antibiotic is 1000x (which it should be) add 5uL antibiotic to your tube. Make sure you add the right one!
- Take a tip on a p200 and lightly touch a colony.
- Eject the tip into the culture and put the cap on.
- For a miniprep, grow overnight for 12-16 hours NOT LONGER.
REMEMBER TO USE PROPER STERILE TECHNIQUE
Adapted from [http://openwetware.org/wiki/Bacterial_cell_culture OpenWetWare]
