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Team:NTNU Trondheim/Biobricks
From 2011.igem.org
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| pBAD||Inducible pBad/araC promoter||BBa_I0500||pSB2K3||pBad is an E. coli promoter that is tightly controlled by:inducer: L-arabinose. Repressor: AraC apparently acts as the repressor. | | pBAD||Inducible pBad/araC promoter||BBa_I0500||pSB2K3||pBad is an E. coli promoter that is tightly controlled by:inducer: L-arabinose. Repressor: AraC apparently acts as the repressor. | ||
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| - | | pBAD2||Alternative pBAD/araC||BBa_K113009||pSB1A2||Says BBa_I0050 doesn | + | | pBAD2||Alternative pBAD/araC||BBa_K113009||pSB1A2||Says BBa_I0050 doesn't work, made new pBAD/AraC |
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Revision as of 20:19, 11 July 2011
BioBricks we have used in our project so far
| Short | BioBrick | Part number | Plasmid | Comment |
| P1 | rrnB P1 promoter | BBa_K112118 | BBb1 entry vector | Contains the rrnB P1 promoter, possibly also junk. Is in BBb format, not BBa, hence not compatible with other parts we are using. Repressible by ppGpp. |
| lambdaPR | lambda pR mod | BBa_R0051 | pSB1A2 | Strong promoter, based on lambda pR, repressible by ci, possibly ppGpp |
| RFP | TetR + p(TetR)+RFP | BBa_K092600 | pSB1A2 | RFP inhibited by tetR, RFP produced by neg. Promoter on TetR |
| Lux | TetR + p(TetR)+RFP+luxI | BBa_K092700 | pSB1A2 | Same as BBa_K092600, but with luxI in addition |
| lacP | lac Promoter (lacI regulated, lambda pL hybrid) | BBa_R0011 | pSB1A2 | Strong promoter, will be on in strains without lacI, off in strains that are lacIq and medium in strains that are lacI |
| lacI | lacI repressor from E. coli (+LVA) | BBa_C0012 | pSB1A2 | Coding region for the LacI protein with an LVA degradation tail and without an RBS. The LVA tag has been added for quicker degradation. Incompatible with systems containing LacI, lactose, or IPTG |
| mC | mCherry, bacterial with RBS and forward terminator | BBa_J06702 | pSB1A2 | mCherry, bacterial with RBS and forward terminator |
| lacI | lacI repressor with RBS | BBa_J24679 | pSB1A2 | RBS added to LacI protein coding region |
| TERM | Double terminator | BBa_B0015 | pSB1AK3 | Double terminator, wide usage. |
| Strong RBS + LacI(LVAtag) + double terminator | BBa_K292006 | pSB1A2 ? | This part contains a strong ribosome binding site, the LacI repressor and a double terminator (BBa_B0030 + BBa_C0012 + BBa_B0014). This brick comes from the BBa_K292005. | |
| GFP | GFP(+LVA) | BBa_K082003 | pSB1AK3 | GFP with degradation LVA tag |
| RBS | Ribosome Binding Site (strong) | BBa_B0034 | pSB1A2 | Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. Brukes i masse forskjellige constructs |
| TERM2 | Double terminator | BBa_B0014 | pSB1AK3 | |
| pBAD | Inducible pBad/araC promoter | BBa_I0500 | pSB2K3 | pBad is an E. coli promoter that is tightly controlled by:inducer: L-arabinose. Repressor: AraC apparently acts as the repressor. |
| pBAD2 | Alternative pBAD/araC | BBa_K113009 | pSB1A2 | Says BBa_I0050 doesn't work, made new pBAD/AraC |
