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Team:Freiburg/Notebook/8 June
From 2011.igem.org
(Difference between revisions)
(→Protein modelling and amplification of parts) |
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| Line 17: | Line 17: | ||
|BBa_K098995 | |BBa_K098995 | ||
|P3, 1E | |P3, 1E | ||
| - | | | + | |amp |
|heat sensitive cI QPI with high promoter | |heat sensitive cI QPI with high promoter | ||
|- | |- | ||
| Line 24: | Line 24: | ||
|amp | |amp | ||
|Lambda phage lysis device - no promoter, This part is in BBb Format.<br/>It is flanked by BamHI and BglII sites instead of XbaI and SpeI. | |Lambda phage lysis device - no promoter, This part is in BBb Format.<br/>It is flanked by BamHI and BglII sites instead of XbaI and SpeI. | ||
| + | |- | ||
| + | |pSB1K3 | ||
| + | |P1, 5A | ||
| + | |kana | ||
| + | | | ||
| + | |- | ||
| + | |pSB1A3 | ||
| + | |P1, 1G | ||
| + | |amp | ||
| + | | | ||
| + | |- | ||
| + | |pSB1C3 | ||
| + | |P1,3A | ||
| + | |chloramphenicol | ||
| + | | | ||
| + | |- | ||
| + | |pSB1T3 | ||
| + | |P1, 7A | ||
| + | |tet | ||
| + | | | ||
| + | |- | ||
| + | |J23104 | ||
| + | |P1, 18K | ||
| + | | | ||
| + | | | ||
|} | |} | ||
Revision as of 10:42, 8 July 2011
Protein modelling and amplification of parts
Investigators:
Protein modelling with pymol: Rüdiger
Amplification of different parts: Julia and Jacob
antibiotics plates:
1oo mg/ml Ampizilin (100 microliter per plate)
1oo mikroliter H2O + 2 microliter Cm
1oo mikroliter H2O + 40 microliter Tet
1oo mikroliter H2O + 10 microliter Kan
| part | location(p=plate) | resistance | info |
| BBa_K098995 | P3, 1E | amp | heat sensitive cI QPI with high promoter |
| BBa_K112022 | P3, 24E | amp | Lambda phage lysis device - no promoter, This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. |
| pSB1K3 | P1, 5A | kana | |
| pSB1A3 | P1, 1G | amp | |
| pSB1C3 | P1,3A | chloramphenicol | |
| pSB1T3 | P1, 7A | tet | |
| J23104 | P1, 18K |
