Team:Grenoble/Projet/Biobricks

From 2011.igem.org

(Difference between revisions)
Line 69: Line 69:
-
                <div class="blocbackground">
+
<div class="blocbackground">
-
 
+
<table align="center">
-
                  <table align="center">
+
<tr>
-
<tr>
+
<td>
-
  <td>
+
<p align="left">
-
      <p align="left">
+
<center><h5>MerR-del (K545103)</h5></center><br/><br/>
-
      <center><h5>MerR-del (K545103)</h5></center><br/><br/>
+
-
 
+
MerR (BBa_K346001) without RBS<br/>
-
      MerR (BBa_K346001) without RBS<br/>
+
PCR gel<br/><br/>
-
      PCR gel<br/><br/>
+
-
 
+
Well 1: Positive control (pSB4K5)<br/>
-
      Well 1: Positive control (pSB4K5)<br/>
+
Well 2: Negative control (Without DNA)<br/>
-
      Well 2: Negative control (Without DNA)<br/>
+
Wells 3, 4, 5, 6, 7: MerR-del (band expected at 740pb, obtained around 700-800pb)<br/><br/>
-
      Wells 3, 4, 5, 6, 7: MerR-del (band expected at 740pb, obtained around 700-800pb)<br/><br/>
+
-
 
+
Confirmed by sequencing<br/>
-
      Confirmed by sequencing<br/>
+
</p>
-
      </p>
+
</td>
-
  </td>
+
<td>
-
 
+
<center><img src="https://static.igem.org/mediawiki/2011/f/fe/01.png"/></center>
-
  <td>
+
</td>
-
<center><img src="https://static.igem.org/mediawiki/2011/f/fe/01.png"/></center>
+
</tr>
-
  </td>
+
<tr>
-
</tr>
+
<td>
-
 
+
<p align="left">
-
<tr>
+
<center><h5>pMerT-GFP (K545002)</h5></center><br/><br/>
-
  <td>
+
-
    <p align="left">
+
Original parts: K346002, E0240<br/>
-
    <center><h5>pMerT-GFP (K545002)</h5></center><br/><br/>
+
PCR gel<br/><br/>
-
 
+
-
    Original parts: K346002, E0240<br/>
+
Well 1: MW<br/>
-
    PCR gel<br/><br/>
+
Well 2: empty well<br/>
-
 
+
Well 3 and 4: clonning failure<br/>
-
    Well 1: MW<br/>
+
Well 5: pMerT-GFP (expected at 1200pb obtained right above 1000pb)<br/><br/>
-
    Well 2: empty well<br/>
+
-
    Well 3 and 4: clonning failure<br/>
+
Confirmed by sequencing<br/>
-
    Well 5: pMerT-GFP (expected at 1200pb obtained right above 1000pb)<br/><br/>
+
</p>
-
 
+
</td>
-
    Confirmed by sequencing<br/>
+
<td>
-
    </p>
+
<center><img src="https://static.igem.org/mediawiki/2011/d/d9/02.png"/></center>
-
  </td>
+
</td>
-
 
+
</tr>
-
  <td>
+
<tr>
-
<center><img src="https://static.igem.org/mediawiki/2011/d/d9/02.png"/></center>
+
<td>
-
  </td>
+
<p align="left">
-
</tr>
+
<center><h5>pCin-CrtEBI (K545000)</h5></center><br/><br/>
-
 
+
-
<tr>
+
Original parts: R0077, K274100<br/>
-
  <td>
+
PCR gel<br/><br/>
-
    <p align="left">
+
-
    <center><h5>pCin-CrtEBI (K545000)</h5></center><br/><br/>
+
Wells 1, 2 and 3: pCin-CrtEBI (Expected at 3500pb, obtained between 3000 and 4000pb)<br/>
-
 
+
Well 4: MW<br/><br/>
-
    Original parts: R0077, K274100<br/>
+
-
    PCR gel<br/><br/>
+
Confirmed by sequencing<br/>
-
 
+
</p>
-
    Wells 1, 2 and 3: pCin-CrtEBI (Expected at 3500pb, obtained between 3000 and 4000pb)<br/>
+
</td>
-
    Well 4: MW<br/><br/>
+
<td>
-
 
+
<center><img src="https://static.igem.org/mediawiki/2011/9/9a/03.png"/></center>
-
    Confirmed by sequencing<br/>
+
</td>
-
  </td>
+
</tr>
-
 
+
<tr>
-
  <td>
+
<td>
-
<center><img src="https://static.igem.org/mediawiki/2011/9/9a/03.png"/></center>
+
<p align="left">
-
  </td>
+
<center><h5>pConst-RBS-CinR (K545004)</h5></center><br/><br/>
-
</tr>
+
-
 
+
Original parts:  J23119, B0034 C0077<br/>
-
<tr>
+
Restriction gel<br/><br/>
-
  <td>
+
-
    <p align="left">
+
Wells 1 and 2: pConst-CinR(expected at 950pb)<br/>
-
    <center><h5>pConst-RBS-CinR (K545004)</h5></center><br/><br/>
+
Wells 4 and 5: cloning failure<br/>
-
 
+
Wells 3 and 6: empty wells<br/>
-
    Original parts:  J23119, B0034 C0077<br/>
+
plasmids were obtained for each well at the expected size,about 2000pb.<br/><br/>
-
    Restriction gel<br/><br/>
+
-
 
+
Confirmed by sequencing<br/>
-
    Wells 1 and 2: pConst-CinR(expected at 950pb)<br/>
+
</p>
-
    Wells 4 and 5: cloning failure<br/>
+
</td>
-
    Wells 3 and 6: empty wells<br/>
+
<td>
-
    plasmids were obtained for each well at the expected size,about 2000pb.<br/><br/>
+
<center><img src="https://static.igem.org/mediawiki/2011/9/96/04.png"/></center>
-
 
+
</td>
-
    Confirmed by sequencing<br/>
+
</tr>
-
  </td>
+
<tr>
-
 
+
<td>
-
  <td>
+
<p align="left">
-
<center><img src="https://static.igem.org/mediawiki/2011/9/96/04.png"/></center>
+
<center><h5>pLac-CinR (S04607)</h5></center><br/><br/>
-
  </td>
+
-
</tr>
+
Original parts: R0011, E0240<br/>
-
 
+
Restriction gel<br/><br/>
-
<tr>
+
-
  <td>
+
<center><h5>pConst-GFP (K545001)</h5></center><br/><br/>
-
    <p align="left">
+
-
    <center><h5>pLac-CinR (S04607)</h5></center><br/><br/>
+
Original parts: J23119, E0240<br/>
-
 
+
Restriction gel<br/><br/>
-
    Original parts: R0011, E0240<br/>
+
-
    Restriction gel<br/><br/>
+
Well 1: pLac-CinR (expected at 950pb)<br/>
-
 
+
Well 2: pConst-CinR (expected at 900pb)<br/>
-
    <center><h5>pConst-GFP (K545001)</h5></center><br/><br/>
+
plasmids were obtained for each well at the expected size,about 2000pb.<br/><br/>
-
 
+
-
    Original parts: J23119, E0240<br/>
+
Confirmed by sequencing<br/>
-
    Restriction gel<br/><br/>
+
</p>
-
 
+
</td>
-
    Well 1: pLac-CinR (expected at 950pb)<br/>
+
<td>
-
    Well 2: pConst-CinR (expected at 900pb)<br/>
+
<center><img src="https://static.igem.org/mediawiki/2011/7/79/05.png"/></center>
-
    plasmids were obtained for each well at the expected size,about 2000pb.<br/><br/>
+
</td>
-
 
+
</tr>
-
    Confirmed by sequencing<br/>
+
<tr>
-
  </td>
+
<td>
-
 
+
<p align="left">
-
  <td>
+
<center><h5>RBS-CinR (S04602)</h5></center><br/><br/>
-
<center><img src="https://static.igem.org/mediawiki/2011/7/79/05.png"/></center>
+
-
  </td>
+
Original parts: B0034, C0077<br/>
-
</tr>
+
PCR gel<br/><br/>
-
 
+
-
<tr>
+
Well 1: RBS-CinR (expected at 1000)<br/>
-
  <td>
+
Wells 2, 3 and 4: clonning failure<br/><br/>
-
    <p align="left">
+
-
    <center><h5>RBS-CinR (S04602)</h5></center><br/><br/>
+
Confirmed by sequencing<br/>
-
 
+
</p>
-
    Original parts: B0034, C0077<br/>
+
</td>
-
    PCR gel<br/><br/>
+
<td>
-
 
+
<center><img src="https://static.igem.org/mediawiki/2011/5/5f/06.png"/></center>
-
    Well 1: RBS-CinR (expected at 1000)<br/>
+
</td>
-
    Wells 2, 3 and 4: clonning failure<br/><br/>
+
</tr>
-
 
+
<tr>
-
    Confirmed by sequencing<br/>
+
<td>
-
  </td>
+
<p align="left">
-
 
+
<center><h5>RBS-TetR (S04603)</h5></center><br/><br/>
-
  <td>
+
-
<center><img src="https://static.igem.org/mediawiki/2011/5/5f/06.png"/></center>
+
Original parts: B0034, C0040<br/>
-
  </td>
+
PCR gel<br/><br/>
-
</tr>
+
-
 
+
well 1: RBS-TetR (expected at 950pb)<br/><br/>
-
<tr>
+
-
  <td>
+
Confirmed by sequencing<br/>
-
    <p align="left">
+
</p>
-
    <center><h5>RBS-TetR (S04603)</h5></center><br/><br/>
+
</td>
-
 
+
<td>
-
    Original parts: B0034, C0040<br/>
+
<center><img src="https://static.igem.org/mediawiki/2011/a/af/07.png"/></center>
-
    PCR gel<br/><br/>
+
</td>
-
 
+
</tr>
-
    well 1: RBS-TetR (expected at 950pb)<br/><br/>
+
</table>
-
 
+
-
    Confirmed by sequencing<br/>
+
-
  </td>
+
-
 
+
-
  <td>
+
-
<center><img src="https://static.igem.org/mediawiki/2011/a/af/07.png"/></center>
+
-
  </td>
+
-
</tr>
+
-
 
+
-
</table>
+
-
              </div>
+
-
 
+
</div>
</div>
</div>
</div>
-
<!--
+
</div>
-
</div>
+
-
-->
+
-
+
-
</div>
+
-
</div>
 
</html>
</html>
{{:Team:Grenoble/Design/pied}}
{{:Team:Grenoble/Design/pied}}

Revision as of 00:04, 29 October 2011

Grenoble 2011, Mercuro-Coli iGEM


Si vous éditez la page commencez par décommenter ces lignes, publier, PUIS commencer à faire ce que vous avez à faire et quand vous avez fini de publier remettez en commentaire. Ne laissez pas le carré trop longtemps si vous n'éditez pas, chaque fois reprenez ce qui est sur internet plutôt que ce que vous avez sur votre PC

Biobricks

Here you can find all our news and composite parts we send to the registry.

<groupparts>iGEM011 Grenoble</groupparts>

You can also find in the registry some of our intermediate constructions:

BBa_S04602

RBS-CinR, BBa_B0034+BBa_C0077

BBa_S04603

RBS-TetR, BBa_B0034+BBa_C0040

BBa_S04606

RBS-TetR-RBS-CinR, BBa_B0034+BBa_C0040+BBa_B0034+BBa_C0077
BBa_S04605

RBS-TetR-RBS-GFP, BBa_B0034+BBa_C0077+BBa_E0240
BBa_S04607

pLac-CinR, BBa_R0011+BBa_C0077
BBa_S04618

pLac-CinR, BBa_K545006+BBa_I13401

MerR-del (K545103)


MerR (BBa_K346001) without RBS
PCR gel

Well 1: Positive control (pSB4K5)
Well 2: Negative control (Without DNA)
Wells 3, 4, 5, 6, 7: MerR-del (band expected at 740pb, obtained around 700-800pb)

Confirmed by sequencing

pMerT-GFP (K545002)


Original parts: K346002, E0240
PCR gel

Well 1: MW
Well 2: empty well
Well 3 and 4: clonning failure
Well 5: pMerT-GFP (expected at 1200pb obtained right above 1000pb)

Confirmed by sequencing

pCin-CrtEBI (K545000)


Original parts: R0077, K274100
PCR gel

Wells 1, 2 and 3: pCin-CrtEBI (Expected at 3500pb, obtained between 3000 and 4000pb)
Well 4: MW

Confirmed by sequencing

pConst-RBS-CinR (K545004)


Original parts: J23119, B0034 C0077
Restriction gel

Wells 1 and 2: pConst-CinR(expected at 950pb)
Wells 4 and 5: cloning failure
Wells 3 and 6: empty wells
plasmids were obtained for each well at the expected size,about 2000pb.

Confirmed by sequencing

pLac-CinR (S04607)


Original parts: R0011, E0240
Restriction gel

pConst-GFP (K545001)


Original parts: J23119, E0240
Restriction gel

Well 1: pLac-CinR (expected at 950pb)
Well 2: pConst-CinR (expected at 900pb)
plasmids were obtained for each well at the expected size,about 2000pb.

Confirmed by sequencing

RBS-CinR (S04602)


Original parts: B0034, C0077
PCR gel

Well 1: RBS-CinR (expected at 1000)
Wells 2, 3 and 4: clonning failure

Confirmed by sequencing

RBS-TetR (S04603)


Original parts: B0034, C0040
PCR gel

well 1: RBS-TetR (expected at 950pb)

Confirmed by sequencing

logo iGEM
iGEM 2011 Main Page


Contact Us:

Clic here !

Retrieved from "http://2011.igem.org/Team:Grenoble/Projet/Biobricks"